Scientific teams and projects

The Bio-electromagnetism Research Group focuses on the development of therapeutic and diagnostic methods that leverage the interaction between electromagnetic fields and biological systems. Our goal is to push the boundaries of medical technologies to make them effective, safe, and accessible. Our team consists of experts and students across various levels of study – we bridge research with clinical practice and contribute to improving the quality of healthcare.

Academic Staff: prof. Dr.-Ing. Jan Vrba, M.Sc., prof. Ing. David Vrba, Ph.D., doc. Ing. Ondřej Fišer, Ph.D., Ing. Tomáš Dřížďal, Ph.D., Ing. Marek Novák, Ph.D., Ing. Tomáš Pokorný, Ph.D.

Ph.D. Students: Ing. Matouš Brunát, Ing. Jan Rédr, Ing. Michaela Černá, Ing. Hana Laierová, Ing. Lukáš Malena, Ing. Jakub Kollár, Ing. Zdeněk Linha

Department of Biomedical Engineering, Nam. Sitna 3105, Kladno, 272 01

For the latest information, please visit our website bioem.fbmi.cvut.cz/doku.php/team 

Below is a selection of methods the team is working on.

Microwave Hyperthermia

Microwave hyperthermia significantly enhances the effectiveness of radiotherapy and chemotherapy in the treatment of cancer. This method allows for reduced doses of conventional treatments, thereby minimizing their side effects.

We are working on the development of a clinical system for microwave hyperthermia focused on the treatment of tumors in the pelvic area, brain, and head and neck. Our work includes:

• Numerical modeling of electromagnetic fields and temperature distribution,
• Development of algorithms for individualized treatment planning,
• Design of applicators, generators, and other key components of the system.

Microwave Non-invasive Temperature Monitoring during Hyperthermia or Tissue Ablation

Accurate temperature monitoring during microwave hyperthermia is crucial for ensuring the safety and effectiveness of treatment. Invasive measurements provide only point-based information, while non-invasive methods, such as magnetic resonance, are financially and technically demanding.

Therefore, we are developing a system for non-invasive temperature monitoring as part of a comprehensive hyperthermic solution. The research includes:

• Numerical simulations and experimental measurements,
• Reconstruction of temperature rise distribution from S-parameters and calculated fields,
• Development of models for the temperature dependence of dielectric properties.

We focus on temperature monitorin in the pelvic area, head&neck, and brain, with a particular emphasis on hyperthermia for glioblastomas. For this reason, we are intensively studying the temperature dependence of dielectric parameters in brain tissue, which is crucial for the accuracy of models and monitoring systems.

Microwave System for Detection and Classification of Stroke

Currently, there is no reliable system for pre-hospital detection and differentiation of stroke types (ischemic vs. hemorrhagic). Rapid diagnosis is crucial for initiating the correct treatment and minimizing permanent consequences.

Our team is developing a compact, portable microwave system in the form of a helmet that allows:

• Early detection and classification of stroke type,
• Monitoring disease progression through differential imaging of changes over time,
• Determining the position and size of the affected area of brain tissue.

The goal is to create a device suitable for use in the field, at emergency departments, and in ambulances, which will significantly speed up the decision-making process for the next steps in treatment.

Measurement of Tissue Dielectric Parameters and Phantom Development

Accurate knowledge of tissue dielectric parameters is essential for all our applications – from hyperthermia to microwave-based diagnostics.

We are developing an affordable measurement system that combines a coaxial probe and a vector network analyzer, aiming to enable rapid evaluation of biological samples, such as immediately after a biopsy. In parallel, we are working on creating tomographic maps of dielectric properties using the MRI-EPT method.

Our activities also include:

• Tissue and anatomical structure segmentation from CT and MRI data,
• Creation of multi-tissue 3D models of the head, pelvis, and whole body,
• Production of realistic biological tissue phantoms with anatomical and dielectric fidelity.

These models and phantoms play a key role in testing and calibrating our therapeutic and diagnostic systems.

Use of Radar Methods in Medicine

We are focused on the development of radar technologies for medical applications, particularly in areas where non-invasive and contactless detection or real-time imaging is crucial. Radar methods represent a promising tool in several clinical and home-based applications:

• Contactless monitoring of vital signs – detection of respiratory rate and heart rate in patients without physical contact, used in intensive care or home settings.
• Navigation systems for catheter insertion – radar imaging assists in the precise real-time guidance of catheters without the need for ionizing radiation.
• Real-time monitoring of tumor ablation – non-invasive monitoring of the ablation process in 3D space, with the ability to precisely register and evaluate the volume of tissue affected by the procedure directly in the patient’s body.
• Imaging of metal projectile position (e.g., gunshot wounds) – rapid localization of fragments or projectiles within the body without the need for CT or X-ray imaging.
• Fall detection in elderly people – development of a radar system for automatic fall detection in home settings and automated assistance alerts.

Electroporation

We focus on the research and development of electroporation technologies, a process that temporarily increases the permeability of cell membranes using an electric field. Electroporation has a wide range of applications in clinical practice, such as in gene therapy, cancer treatment, targeted drug delivery, and biotechnology.

Our activities include:

• Study of electroporation effects on target tissue – optimizing parameters to ensure that the intervention is both effective and gentle.
• Analysis of electroporation effects on surrounding tissue in the human body – particularly monitoring undesirable effects, such as hemolysis during cardiac ablation.
• Numerical simulations of physical phenomena during electroporation – modeling the distribution of electric fields and temperature changes during procedures in clinical practice.
• Development of device and electrodes – designing new types of electrodes and control units for clinical and laboratory use.
• Gene transfection – exploring methods for transporting genetic material into cells using electroporation, especially for gene therapy purposes.

 
Low-field Magnetic Resonance Imaging

As part of the European project "Affordable low-field MRI reference system" by the EURAMET agency, we are collaborating with research institutions on the development of low-cost magnetic resonance imaging systems with a B₀ = 50 mT.

The system being developed at the faculty will enable imaging of the human head and limbs. The magnetic field will be generated by a Halbach magnet composed of approximately 2500 neodymium permanent magnets – as a result, the device will be:

• Significantly smaller and lighter than current clinical MRI scanners,
• Completely passive, and thus with low operating costs,
• Safer for patients with implants.

LF MRI systems represent a potentially affordable alternative for diagnostics, particularly in resource-limited environments.

Assessment of Blood Flow and 3D Printing of Hearts

For the purpose of planning interventional cardiology procedures, we perform segmentation of cardiac structures from CT images and create detailed 3D models of the heart. In these models, we identify and design up to six possible access points for puncturing the interatrial septum, particularly in relation to the closure of the left atrial appendage (LAA) – a procedure that plays a key role in reducing the risk of stroke in patients with atrial fibrillation.

Simultaneously, in the context of evaluating this risk, we conduct numerical analysis of flow ratios in the left atrium to explore the impact of the LAA morphology on thrombus formation and, thus, the likelihood of embolic events.

The heart models are:

• Printed using FDM technology from soft plastics,
• Used for planning the optimal procedure,
• Also serve for simulating blood flow and assessing the risk of thrombus formation around the LAA.

Tonagena, s.r.o. - CTU Spin-off 

In 2023, the Bioelectromagnetism team founded the CTU spin-off, Tonagena s.r.o., to commercialize the developed technologies and devices. The team is actively involved in all aspects of product launch, from development and testing to certification, production, and sales. Since the foundation of the spin-off, the TonaPulse® system, including a unique electrode system for adherent cell cultures, has been commercialized. This system is already being used, for example, at the 3rd Faculty of Medicine, Charles University, and the 2nd Faculty of Medicine, Charles University, for research on irreversible electroporation for the treatment of cardiac arrhythmias, as well as for gene transfection research, which holds promise for treating cancers or currently incurable genetic diseases.

Research with the system developed by the spin-off has already demonstrated potential undesirable effects of irreversible electroporation, such as hemolysis.

In addition to electroporation, Tonagena s.r.o. is involved in custom development of microwave applicators and radar solutions for significant local companies, where it has already delivered hardware in both areas.

More information is available at tonagena.cz.

Projects for Students

Every year, we offer semester and year-long projects, which serve as the foundation for bachelor’s and master’s theses in the areas of our research. If you are interested in contributing to the promising applications mentioned above, feel free to contact us.

Additionally, we organize a student scientific conference REMMA (Research in ElectroMagnetic Medical Applications), where the main goal is to support students in creating their own scientific outputs in the form of conference contributions. This provides an opportunity to discuss their scientific work with a broader community and contribute to the creation of high-quality, impactful publications.

Collaboration

Our research activities are conducted as part of prestigious international projects and in collaboration with university hospitals, scientific centers and industrial partners around the world.

• Eledia Research Center, Trento, Italy
• Erasmus MC Cancer Institute, Rotterdam, Netherlands
• Center for Cure and Care, Eindhoven University of Technology, Eindhoven, Netherlands
• MR, Biology and Applied Physics, General Electric Global Research Center, Niskayuna, USA
• Charles University - 3rd Faculty of Medicine
• Faculty of Electrical Engineering, Czech Technical University in Prague
• Institute of Photonics and Electronics, Czech Academy of Sciences
• ALBA – RESTEK Group, Italy
• BTL
• Physikalisch-Technische Bundesanstalt (PTB), Berlin, Germany
• Microbiological Institute, Czech Academy of Sciences
• Chalmers University of Technology, Gothenburg, Sweden
• University of Maryland, Division of Translational Radiation Sciences, USA

Funding of Research

Funding for our research activities is secured through research projects supported by various grant agencies, as well as through commercial contracts with industrial partners.

Selected current and recently completed research projects:

Interreg Central Europe (2024-2026) “Microwave imaging technology transfer to innovate the medical sector”, CE0200670 – MedWaveImage, CTU is a project partner.

EURAMET (2024-2026) “Affordable, Accessible, Adjustable and Accurate mr Imaging”, 22HLT02 A4IM, CTU is a project partner.

GA ČR (2021-2023) “Multiphysical Study of Superposition of Electromagnetic Waves in Human Head Model to Verify the Feasibility of Microwave Hyperthermia of Brain Tumors”, Standard project, GA21-00579S, Prof. David Vrba was PI.

GA ČR (2020-2022): “Electrically-read quantum diamond sensors for nuclear magnetic resonance and chemical sensing”, Standard project, GA20-28980S, Prof. Nesládek was PI.

MŠMT (2019-2022): “Development of metamaterial applicators for the regional hyperthermia system and evaluation of the accuracy of treatment planning algorithms”, Project No.LTC19031. MŠMT LT - INTER-EXCELLENCE, Prof. Jan Vrba was PI.

H2020 (2019-2020): “Left atrial appendage electrical Isolation via bio-photonic optical confirmation to treat persistent atrial fibrillation”, Fast Track to Inovation, CTU was a project partner.

More information can be found on the website bioem.fbmi.cvut.cz/doku.php/projects 

Publications in Impact Factor Journals 

Selected Publications 2021 - 2025:

1.    Linha, Z., Vrba, J., Kollar, J., Fiser, O., Pokorny, T., Novak, M., Drizdal, T., Vrba, D. An Inexpensive System for Measuring the Dielectric Properties of Biological Tissues Using an Open-Ended Coaxial Probe, IEEE Transactions on Instrumentation and Measurement, 2025, doi: 10.1109/TIM.2025.3561426.
2.    Kollar, J., Novak, M., Babak, B., Drizdal, T., Vrba, J., Vrba, D., Pokorny, T., Linha, Z., Fiser, O. Potential of UWB Radar Systems in Monitoring Liver Ablation: A Phantom Model Study, IEEE Transactions on Antennas and Propagation, 2025, ISSN 0018-926X, doi: 10.1109/TAP.2024.3513555.
3.    Malena, L.; Fišer, O.; Dřížďal, T.; Pokorný, T.; Novák, M.; Vrba, J.; Vrba, D. Feasibility of Glucose Concentration Estimation in Whole Blood Samples Using Noninvasive Metamaterial Microwave Sensor, IEEE Sensors Journal. 2025, 25(5), 8259-8268. ISSN 1530-437X.
4.    Pokorný, T.; Vrba, D.; Fišer, O.; Salucci, M.; Vrba, J., Systematic Optimization of Training and Setting of SVM-Based Microwave Stroke Classification: Numerical Simulations for 10 Port System, IEEE Journal of Electromagnetics, RF and Microwaves in Medicine and Biology. 2024, 8(3), 273-281. ISSN 2469-7249.
5.    Dřížďal, T.; van Rhoon, G.C.; Fišer, O.; Vrba, D.; van Holthe, N.; Vrba, J.; Paulides, M.M., Assessment of the thermal tissue models for the head and neck hyperthermia treatment planning, JOURNAL OF THERMAL BIOLOGY. 2023, 115 ISSN 0306-4565.
6.    Fišer, O.; Hrubý, V.; Vrba, J.; Dřížďal, T.; Tesařík, J.; Vrba, J.; Vrba, D., UWB Bowtie Antenna for Medical Microwave Imaging Applications, IEEE Transactions on Antennas and Propagation. 2022, 70(7), 5357-5372. ISSN 0018-926X.
7.    Vrba, D.; Malena, L.; Albrecht, J.; Fricova, J.; Anders, M.; Rokyta, R.; Rodrgues, D.; Vrba, J., Numerical Analysis of Transcranial Magnetic Stimulation Application in Patients With Orofacial Pain, IEEE Transactions on Neural Systems and Rehabilitation Engineering. 2022, 30(4.4.2022), 590-599. ISSN 1534-4320.
8.    Vrba, J.; Janča, R.; Bláha, M.; Kršek, P.; Vrba, D., Novel Paradigm of Subdural Cortical Stimulation Does Not Cause Thermal Damage in Brain Tissue: A Simulation-Based Study IEEE Transactions on Neural Systems and Rehabilitation Engineering. 2021, 29(1.3.2021), 230-238. ISSN 1534-4320.

Other selected publications can be found on the website bioem.fbmi.cvut.cz/doku.php/publication, or by author in the Web of Science or Scopus databases.
 

Department of Biomedical Technology, Sitna 3105, 272 01 Kladno

Assoc. Prof. Roman Matějka, Ph.D.

Who we are?

We are one of the teams of the Department of Biomedical Technology, which deals with the development of bioreactors (devices in which the cells, tissues or organs are developed in vitro), bioprinting and the development of artificial tissues and organs using these methods.
By combining different materials (e.g. collagen hydrogel, decellularised blood vessel support) and different cell types, we are able to create a customized organ or tissue replacement. We are thus linking the fields of physiology and mechanical engineering. Our outputs are a collaboration of several institutions with which we have close ties (CAS, IKEM, Biohealing, National Cell and Tissue Centre etc.).

Members:

Assoc. Prof. Roman Matějka, Ph.D., Jana Matějková, Ph.D, Denisa Kaňoková

What research do we do?

NW24J-02-00061 Vascular prostheses made from decellularized cadaveric donor tissues modified with stem cells and 3D bioprinting

Cardiovascular surgery suffers from a shortage of suitable biomaterials for vascular bypasses and patches. Autologous grafts (the patient's own healthy blood vessels that are implanted in place of damaged vessels) such as the vena saphena or the arteria mammaria interna are the gold standard. However, their availability is limited and they burden the patient with possible complications from graft harvesting. Synthetic substitutes (various knitted materials) are readily available but fail due to thrombosis, intimal hypeplasia and infection. Allogeneic grafts (transplanted vessels from other patients) are more resistant to infection but have poor long-term patency and require immuno-suppressive therapy. Available chemically stabilised and/or decellularised allografts or xenografts (modified blood vessels from other organisms, e.g. domestic pig) do not perform better than synthetic prostheses. The aim is to create a new vascular replacement by tissue engineering methods using a unique combination of existing methods: vascular allografts and xenografts; decellularization; recellularization in a bioreactor with Wharton's jelly mesenchymal stem cells (WJ-MSCs) and cryopreservation. 

NW24-08-00064 Advanced Testing of Novel Thrombolytics: An Innovative Model Using Precise Thrombus Structure and Interaction with the Vascular Wall under Simulated Pathophysiological Conditions

Ischemic stroke represents a major socioeconomic threat worldwide. The cause of this pathology lies in the obstruction of cerebral arteries by thrombus. Although thrombolytic therapy is currently clinically approved, it has a relatively low success rate. Failure of thrombolysis and subsequent limited recanalization of the vessels can be largely attributed to the structure of the thrombus. Unfortunately, the relationship between clot structure, its biomechanical properties, and lytic susceptibility, which are crucial for successful recanalization, has been inadequately explored and largely neglected in the design of thrombolytics. 

What's it for

The field of tissue engineering and regenerative medicine is a rapidly developing field that has increasing potential given the ageing population. The human body is affected by many degenerative diseases as we age, and in the majority of cases the body will heal without external intervention. However, some diseases and injuries require the use of replacements or implants in cases where significant damage to a tissue or organ has occurred. The replacement may be of biological or artificial origin. In some cases, the replacement is taken directly from the patient's body, such as the vena saphena as a bypass of a coronary vessel. In other cases, the replacement may be taken from the body of the donor. Finally, the replacement may be artificial.
Our team's goal is to develop a replacement that fully replaces the original organ and the patient can quickly return to normal life. This simplified process consists of the following steps:

• collection and isolation of stem cells (e.g. from fat, bone marrow, umbilical cord, etc.).
• cell expansion under static conditions
• plating the cells on or into a scaffold - the basis of the future organ
• 3D printing and bioprinting of the scaffold
• decellularization of xenogeneic tissue
• cultivation of the scaffolds with cells in a bioreactor, stimulation and subsequent differentiation of the cells into a given phenotype (e.g. smooth muscle)
• implantation of the created substitute into the patient

Bioreactors are devices in which stem cells are cultured in order to differentiate the cells into a given tissue type, or phenotype. The differentiation of the cells occurs through the action of physico-chemical processes. Chemically, differentiation is promoted by the unique composition of the culture medium, which contains growth factors and other substances suitable for cell growth and differentiation. The physical forces acting on the cells then create a bioreactor. Different types of cells require different types of stimulation - e.g., an endothelium requires flow of the medium, an osteoblast requires hydrostatic pressure, a muscle cell requires stretching combined with hydrostatic pressure, a neuron requires an electrical impulse, etc. At the same time, bioreactors can specifically populate the media with cells (e.g., rotating a circular vascular carrier for uniform populations), continuously deliver nutrients and remove metabolites. Thus, they contribute to the formation of a high quality cell culture, tissue or organ, compared to static conditions, i.e. when no external forces act on the cells except chemical influences from the culture medium.
Creating the optimal carrier for a future tissue or organ is also a current challenge in biomedical engineering. Artificial carriers made of various knitted fabrics and materials often suffer from ailments that lead to health complications and rejection of the material by the patient's body. Therefore, the aim is to find and modify a carrier that is preferably of biological origin, which the patient will not react adversely to and thus naturally incorporate into his body. Our current research focuses on the use of carriers from allogeneic or xenogeneic donors. Allogeneic donors are meant to be human donors, xenogeneic donors are another animal species, i.e. e.g. pig. Before using such material, however, it is necessary to deprive the material of the donor cells, so called decellularizing it, resulting in a matrix of protein material. Again, a different type of bioreactor is used to create such a carrier.
Sometimes, however, artificial materials cannot be avoided. Therefore, we are also involved in the cultivation of cells on biomaterials, where we modify the properties of the materials by selected physical processes and then apply a cell culture to the material, which is further differentiated in the bioreactor. We can then potentially inject this complex into the patient, where a layer of the patient's autologous cells should ensure the resulting biocompatibility and successful engraftment of the new prosthesis.

With whom we cooperate

• St. Anne's University Hospital Brno
• Masaryk University
• Institute of Clinical and Experimental Medicine
• Institute of Rock Structure and Mechanics of the CAS v.v.i.
• Biohealing s.r.o.
• PrimeCell a.s.
• National Tissue and Cell Center a.s.

Selected publications

1.  Matějka, R., M. Koňařík, J. Štěpanovská, J. Lipenský, J. Chlupáč, D. Turek, Š. Pražák, A. Brož, Z. Šimůnková, I. Mrázová, S. Forostyak, P. Kneppo, J. Rosina, L. Bačáková A J. Pirk Bioreactor Processed Stromal Cell Seeding and Cultivation on Decellularized Pericardium Patches for Cardiovascular Use. Applied Sciences,  2020, 10(16). IF 2,68, Q2 ENGINEERING
2. Matejkova, J.; Kanokova, D.; Supova, M.; Matejka, R. A New Method for the Production of High-Concentration Collagen Bioinks with Semiautonomic Preparation. Gels 2024, 10, 66. https://doi.org/10.3390/gels10010066
3. Kanokova, D.; Matejka, R.; Zaloudkova, M.; Zigmond, J.; Supova, M.; Matejkova, J. Active Media Perfusion in Bioprinted Highly Concentrated Collagen Bioink Enhances the Viability of Cell Culture and Substrate Remodeling. Gels 2024, 10, 316. https://doi.org/10.3390/gels10050316
4.  Stepanovska, J., R. Matejka, J. Rosina, L. Bacakova A H. Kolarova Treatments for enhancing the biocompatibility of titanium implants. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub, Mar 2020, 164(1), 23-33., IF 1,25, Q4
5. Stepanovska, J.*, R. Matejka*, M. Otahal, J. Rosina A L. Bacakova The Effect of Various Surface Treatments of Ti6Al4V on the Growth and Osteogenic Differentiation of
6. Chlupac, J.*; Matejka, R.*; Konarik, M.; Novotny, R.; Simunkova, Z.; Mrazova, I.; Fabian, O.; Zapletal, M.; Pulda, Z.; Lipensky, J.F.; Stepanovska, J.; Hanzalek, K.; Broz, A.; Novak, T.; Lodererova, A.; Voska, L.; Adla, T.; Fronek, J.; Rozkot, M.; Forostyak, S.; Kneppo, P.; Bacakova, L.; Pirk, J. Vascular Remodeling of Clinically Used Patches and Decellularized Pericardial Matrices Recellularized with Autologous or Allogeneic Cells in a Porcine Carotid Artery Model. Int. J. Mol. Sci. 2022, 23, 3310, IF 5,92, Q1 BIOCHEMISTRY AND MOLECULAR BIOLOGY, *These authors contributed equally to this work.
7. Chlupac, J., Filova, E., Havlikova, J., Matejka, R., Riedel, T., Houska, M., Brynda, E., Pamula, E., Rémy, M., Bareille, R., Fernandez, P., Daculsi, R., Bourget, C., Bacakova, L., Bordenave, L.  The gene expression of human endothelial cells is modulated by subendothelial extracellular matrix proteins: Short-term response to laminar shear stress (2014) Tissue Engineering - Part A, 20 (15-16), pp. 2253-2264.
8. Procházka, V., Matějka, R., Ižák, T., Szabó, O., Štěpanovská, J., Filová, E., Bačáková, L., Jirásek, V., Kromka, A. Nanocrystalline diamond-based impedance sensors for real-time monitoring of adipose tissue-derived stem cells (2019) Colloids and Surfaces B: Biointerfaces, 177, pp. 130-136.
9. Opatrný, V., Moláček, J., Třeška, V., Matějka, R., Hes, O. Perfusion of a Kidney Graft From a Donor After Cardiac Death Based on Immediately Started Pulsatile Machine Perfusion—An Experimental Study on a Small Animal (2018) Transplantation Proceedings, 50 (5), pp. 1544-1548.
10.  Moláček, J., Opatrný, V., Matějka, R., Baxa, J., Třeška, V. Retrograde oxygen persufflation of kidney - Experiment on an animal (2016) In Vivo, 30 (6), pp. 801-805.
11.  Molacek, J., Opatrný, V., Treska, V., Matejka, R., Hes, O. Možnosti zlepšení vlastností ledvinných štěpů od dárců s rozšířenými kritérii - experimentální studie [Options to improve the quality of kidney grafts from expanded criteria donors experimental study] (2018) Rozhledy v chirurgii : mesicnik Ceskoslovenske chirurgicke spolecnosti, 97 (5), pp. 193-201.
12.  Vondrášek, D., Hadraba, D., Matějka, R., Lopot, F., Svoboda, M., Jelen, K. Uniaxial tensile testing device for measuring mechanical properties of biological tissue with stress-relaxation test under a confocal microscope (2018) Manufacturing Technology, 18 (5), pp. 866-872.
13. Lucie Bacakova, Martina Travnickova, Elena Filova, Roman Matějka, Jana Stepanovska, Jana Musilkova, Jana Zarubova and Martin Molitor (October 10th 2018). The Role of Vascular Smooth Muscle Cells in the Physiology and Pathophysiology of Blood Vessels, Muscle Cell and Tissue - Current Status of Research Field, Kunihiro Sakuma, IntechOpen, DOI: 10.5772/intechopen.77115. Available from: https://www.intechopen.com/books/muscle-cell-and-tissue-current-status-of-research-field/the-role-of-vascular-smooth-muscle-cells-in-the-physiology-and-pathophysiology-of-blood-vessels
14. Lucie Bacakova, Martina Travnickova, Elena Filova, Roman Matejka, Jana Stepanovska, Jana Musilkova, Jana Zarubova and Martin Molitor (October 10th 2018). Vascular Smooth Muscle Cells (VSMCs) in Blood Vessel Tissue Engineering: The Use of Differentiated Cells or Stem Cells as VSMC Precursors, Muscle Cell and Tissue - Current Status of Research Field, Kunihiro Sakuma, IntechOpen, DOI: 10.5772/intechopen.77108. Available from: https://www.intechopen.com/books/muscle-cell-and-tissue-current-status-of-research-field/vascular-smooth-muscle-cells-vsmcs-in-blood-vessel-tissue-engineering-the-use-of-differentiated-cell

Application results

• Matejka R, Stepanovska J, Rosina J, Hruzova D, Zarubova J, Filova E. A cultivation chamber for dynamic cultivation of cells on tubular carriers. Utility model, registered on 13. 12. 2016 under No. PUV 2016-33170, approved on 07. 03. 2017 under No. UV30441. Owners: Czech Technical University, Faculty of Biomedical Engineering, Kladno; National Cell and Tissue Centre, Inc., Brno; Institute of Physiology, Acad. Sci. CR, Prague.
• Matejka R, Stepanovska J, Rosina J, Kneppo P, Brynda E, Riedel T, Filova E, Travnickova M, Zarubova J, Riedelova Z. Cultivation chamber for stimulation of planar decelularized pericardium. Utility model, registered on 27.12.2016 under No. PUV 2016-33237, approved on 30.5.2017 under No. UV 30705. Owners: Czech Technical University, Faculty of Biomedical Engineering, Kladno; Institute of Macromolecular Chemistry, Acad. Sci. CR, Prague; Institute of Physiology, Acad. Sci. CR, Prague.
• Matejka R, Stepanovska J, Rosina J, Hruzova D, Zarubova J. System for rotational endothelialization of vascular prostheses. Utility model, registered on 19. 06. 2017 under No. PUV 2017-33823, approved on 3.10.2017 under No. UV 31066. Owners: Czech Technical University, Faculty of Biomedical Engineering, Kladno; National Cell and Tissue Centre, Inc., Brno; Institute of Physiology, Acad. Sci. CR, Prague.
• Matejka R, Prochazka V, Izak T, Stepanovska J, Kromka A, Travnickova M, Bacakova L. Cultivation chamber for optical-electrical monitoring of biological cultures in-vitro with optical-transparent diamond electrodes. Utility model, registered on 21. 12. 2016 under No. PUV 2016-33219, approved on 18. 05. 2017 under No. UV 30691. Owners: Institute of Physics, Acad. Sci. CR, Prague; Institute of Physiology, Acad. Sci. CR, Prague.
   

Other research projects

• NV18-02-00422 New materials for cardiovascular surgery based on modified decellularised tissues
• NV19-02-00068 Bioartificial cardiovascular patches and vascular replacements based on porcine collagen reinforced nano/microfibres remodelled with stem cells in bioreactors
• 15-29153A Development of a pericardium-based aortic valve using primary and stem cells and mechanical loading in a bioreactor
• TA04011345 Small diameter vascular prostheses populated with endothelial and bone marrow stem cells in a bioreactor
   

Demonstration of selected cultivation systems.

The process of culturing 3D collagen gels with cells

System for the preparation of collagen bioinks

Preparation of a cardiovascular graft for implantation.

Biosignal Recognition & Artificial Inteligence in Neuroscience

The research team is currently involved in the processing and analysis of EEG and PSG records. Measurement of brain electrical activity is used in clinical practice as a diagnostic method. It is used for example in the detection of epilepsy, in the analysis of sleep states and memory consolidation. In addition to clinical practice, EEG is processed and evaluated in research. The research area requires other methods of signal analysis, for example for group measurements. The research team is engaged in processing and analysis of human and animal EEG

Who we are?

Our research team focuses on both basic and applied research into methods and processes for EEG analysis in animal and human subjects.
Assoc. Prof. Ing. Marek Piorecký, Ph.D.: He has a practical background in research and development of original systems and methodologies for computer-aided processing and analysis of biological signals. His work includes the analysis of sleep EEG recordings and other physiological signals such as ECG and respiration. He is also involved in simultaneous EEG-fMRI measurements, including the processing and analysis of the recorded data.

Ing. Václava Piorecká: She specializes in the analysis of animal EEG recordings and the development of tools for evaluating group data in animal experiments. Her work includes sleep EEG analysis, microstate analysis, and sleep acoustic stimulation.

Ing. Filip Černý: He focuses on the analysis of EEG signals using microstate analysis and participates in sleep EEG recording in a collaborative laboratory setting.

Ing. Jan Štrobl, Ph.D.: He works on the removal of artifacts from long-term EEG recordings and provides support for statistical data analysis.

WHAT WE COOPERATE

• Národní ústav duševního zdraví
• Nemocnice Na Bulovce
• Český ústav informatiky, robotiky a kybernetiky

SELECTED PUBLICATIONS

1. Piorecký, M.; Koudelka, V.; Piorecká, V.; Štrobl, J.; Dudysová, D.; Kopřivová, J., Real-Time Excitation of Slow Oscillations during Deep Sleep Using Acoustic Stimulation, Sensors. 2021, 20(15), ISSN 1424-8220.
2. Kliková, M.; Piorecký, M.; Miletínová, E.; Janků, K.; Urbaczka Dudysová, D.; Bušková, J., Objective rapid eye movement sleep characteristics of recurrent isolated sleep paralysis: a case-control study, Sleep. 2021, 44(11), 1-7. ISSN 1550-9109.
3. Štrobl, J.; Viktorin, V.; Piorecký, M.; Griskova-Bulanova, I.; Hubený, J.; Brunovský, M.; Páleníček, T.; Koudelka, V., Unveiling stimulus transduction artifacts in auditory steady-state response experiments: Characterization, risks, and mitigation strategies, Biomedical Signal Processing and Control. 2025, 2025(101), 1-14. ISSN 1746-8094.
4. Dudysová, D.; Janků, K.; Piorecký, M.; Hantáková, V.; Orendáčová, M.; Piorecká, V.; Štrobl, J.; Kliková, M. et al., Closed-loop auditory stimulation of slow-wave sleep in chronic insomnia: a pilot study, Journal of Sleep Research. 2024, 33(6), 1-11. ISSN 1365-2869.
5. Černý, F.; Piorecká, V.; Kliková, M.; Kopřivová, J.; Bušková, J.; Piorecký, M., All-night spectral and microstate EEG analysis in patients with Recurrent Isolated Sleep Paralysis (RISP), Frontiers in Neuroscience. 2024, 18 ISSN 1662-453X.
6. Miletínová, E.; Piorecký, M.; Koudelka, V.; Jiříček, S.; Tomeček, D.; Brunovský, M.; Horáček, J.; Bušková, J., Alterations of sleep initiation in NREM parasomnia after sleep deprivation – A multimodal pilot study, Sleep Medicine: X. 2023, 6 ISSN 2590-1427.
7. Piorecký, M.; Bartoň, M.; Koudelka, V.; Bušková, J.; Kopřivová, J.; Brunovský, M.; Piorecká, V., Apnea Detection in Polysomnographic Recordings Using Machine Learning Techniques, Diagnostics. 2021, 11(12), 1-21. ISSN 2075-4418.
8. Vejmola, Č.; Tylš, F.; Piorecká, V.; Koudelka, V.; Kadeřábek, L.; Novák, T.; Páleníček, T., Psilocin, LSD, mescaline, and DOB all induce broadband desynchronization of EEG and disconnection in rats with robust translational validity, Translational Psychiatry. 2021, 11(1), 1-8. ISSN 2158-3188.
9. Tylš, F.; Vejmola, Č.; Koudelka, V.; Piorecká, V.; Kadeřábek, V.; Bochin, M.; Novák, T.; Kuchař, M. et al., Underlying pharmacological mechanisms of psilocin-induced broadband desynchronization and disconnection of EEG in rats, Frontiers in Neurology. 2023, 17 ISSN 1664-2295.

Department of Information and Communication Technologies in Medicine

José Martího 269/31, 162 52, Praha 6 - Veleslavín

Ing. Pavel Smrčka, Ph.D.

Who we are?

Multidisciplinary research team composed of experts from FBME CTU, CIIRC CTU and 1st Faculty of Medicine, Charles University.
Members?
Pavel Smrčka, Tomáš Veselý, Martin Vítězník, Radim Kliment, Jan Kašpar, Karel Hána, Jan Mužík, Markéta Janatová

What research we do?

Measuring, transmission, on-line processing, imaging, archiving and off-line processing of biological signals in real time. We specialize in research and experimental development of telemetric systems for professional monitoring in stress conditions, eg in athletes, soldiers and firefighters using the wearable electronics. 
The promotional video shots of the scientific team:

• https://www.youtube.com/watch?v=tf-mCrgOOz8&feature=youtu.be
• https://www.youtube.com/watch?v=8FoKc6AAuZQ

Examples of realized systems:

VLV 4  - portable medical polygraphic unit – measuring and on-line Wifi streaming of ECG, breath curve, activity, body temperature, skin resistance from up to 20 persons simultaneously. Includes advanced multiplatform software. The primary use of the device is to support research in biology, psychology, human research etc. The system enables monitoring individual physiological responses to various stimuli. 

FlexiGuard / MOSENZ -  Personal security surveillance system to support training and intervention of Integrated Rescue System units. Enables long-term telemetric monitoring of health-physiological data and the environment in real time – heart rate, body and environmental temperature, physical activity, humidity, body position, breath frequency, blood presssure, SpO2, GPS position. Can monitor up to 50 persons simultaneously and transfer the data up to 2 km.

Who we cooperate with?

• Neurological clinics, Faculty Hospital Motol - doc. MUDr. Jaroslav Jeřábek, CSc.
• Clever Technologies, s.r.o., spin-off company FBME CTU
• CASRI - Scientific and service workplace of physical education and sports
• Technical Univerzity Košice – Department of Aviation
• and many other

Current research projects

• 2024 – present: Augmented reality for remote biomonitoring and rescue operations in CBRN situations, provider: Ministry of the Interior of the Czech Republic, project number: VB02000036
• 2023 - present: Research, development and streamlining of advanced end-of-line measurement and warning systems for population protection, provider: Ministry of the Interior of the Czech Republic, project number: VK01020181
• 2022 – present: Modular multisensory professional clothing for risk management, health and safety protection of IZS members using artificial intelligence methods, provider: Ministry of the Interior of the Czech Republic, project number: VJ02010031

Selected publications:     

Granted patents:

1. Hána, K.; Kašpar, J.; Mužík, J.; Smrčka, P.: Security monitoring system especially for seniors and the method implemented thereon, 2021, Patent CZ 308686.
2. P; Hána, K.; Kašpar, J.; Kučera, L.; Mužík, J.; Smrčka, P.; Veselý, T.; Vítězník, M.: Method for wirelessly connecting a smart home to a rescue system patrol and system for implementing it, 2020, patent CZ 308531.
3. P; Smrčka, P.; Hána, K.; Kašpar, J.; Kneppo, P.; Tyšler, M.: System for measuring biological and technical quantities in a strong and variable electromagnetic field environment, 2019, patent CZ 307752
4. Hána, K.; Kašpar, J.; Kučera, L.; Mužík, J.; Smrčka, P.; Veselý, T.; Vítězník, M.: Surveillance equipment for monitoring persons, especially indifficult conditions and the system of sensor placement on the human body. Patent CZ 307930. 2019-07-17.    
5. Hlavinka, P.; Šebelka, Z.; Hána, K.; Kašpar, J.; Mužík, J.; Smrčka, P.: Method of three-stage communication of the notification center and the end element of the warning. Patent CZ 307931. 2019-07

Recent journal publications:

• Veselý, T. Smrčka, P. Kliment, R. Vítězník, M. Hon, Z. Hána, K:: Accuracy Improvement of Energy Expenditure Estimation Through Neural Networks: A Pilot Study, AI 2024, 5(4), 2914-2925, ISSN 1 2673-2688., WOS: 001384119100001, Q2, IF 3.1
• Kantor, J.; Vilímek, Z.; Vítězník, M.; Smrčka, P.; Campbell, E.A.; Bucharová, M.; Grohmannová, J.; Špinarová, G. et al.: Effect of low frequency sound vibration on acute stress response in university students—Pilot randomized controlled trial, Frontiers in Psychology. 2022, 2022(13), ISSN 1664-1078., WOS: 000875775100001, Q1, IF 3.8
• Janatová, M.; Pětioký, J.; Hoidekrová, K.; Veselý, T.; Hána, K.; Smrčka, P. ; Štěpánek, L.; Lippert-Grunnerová, M. et al.: System for Game-like Therapy in Balance Issues Using Audiovisual Feedback and Force Platform, Electronics. 2022, 11(8), ISSN 2079-9292., WOS: 000785148900001, Q2, IF: 2.9
• Veselý, T.; Janatová, M.; Smrčka, P; Vítězník, M.; Kliment, R.; Hána, K. :Measuring of the Energy Expenditure during Balance Training Using Wearable Electronics, Electronics. 2022, 11(7), ISSN 2079-9292. WOS: 000785148900001 , Q2, IF: 2,9
• Šajtárová, L.; Janatová, M.; Veselý, T.; Lopotová, M.; Smrčka, P.; Hána, K.: A randomized controlled study of the effect of balance disorder therapy using audiovisual feedback on senior citizens, Časopis Česká a Slovenská neurologie a neurochirurgie. 2020, 83(1)(20202 (1)), 101-104. ISSN 1210-7859,  WOS:000514084800014, Q4, IF: 0.377

Department of Biomedical Technology, nám. Sítná 3105, Kladno, 272 01

Assoc.Prof. Dr.Rer.Nat. Aleš Tichopád, Ph.D.

The research team CzechHTA has its own website at:

www.czechhta.cz

Who are we?

We are a group focused on research in the field of analyzing large healthcare data and statistical modeling with the aim of better understanding the efficiency of healthcare processes and technologies, including medications. Our work involves exploring the relationships between clinical outcomes and economic aspects. We utilize our knowledge of processing big data and mathematical models to create and evaluate healthcare indicators at the provider level, in defined processes, and even for complex patient pathways within the healthcare system. Other areas of interest for our team include healthcare financing and provision systems, healthcare economics, and management.

What do we do?

• Research and development of methods for processing large healthcare payer data and their applications.
• Research focusing on the economics of new healthcare technologies, including medications.
• Study of healthcare economics and management.
• Teaching in the Master's program in System Integration of Processes in Healthcare.
• Expert activities in the field of procurement and operation of medical equipment.
• Contract research for manufacturers of medical devices and medications, as well as research organizations.

Department of Natural Sciences, nám. Sítná 3105, Kladno, 272 01

Ing. Tomáš Parkman, Ph.D.
Prof. Ing. Miroslava Vrbová, CSc

The XUV laboratory is a scientific research laboratory focused on the development and characterization of table-top soft X-ray sources and their applications in spectroscopy and imaging of biological structures. Soft X-rays (abbreviated SXR) can be used for example for imaging sub-cellular structures with very high resolution in the order of tens of nanometers. SXR microscopes typically operate within the spectral region of the "water window" wavelength (2.3 nm to 4.4 nm). Biological samples do not need to be specially prepared or fluorescent markers used to obtain contrast, as this is obtained by absorption of X-rays by the sample. One of the research directions of our group is the development and optimization and application of two types of laboratory SXR source – pulsed high-current 
Z-pinch discharge plasma and pulsed laser-induced plasma source. A new application of a laser plasma-based SXR laboratory source has also been developed in this laboratory. Very short SXR pulses serve as excitation source for time-resolved luminescence spectroscopy of solid state scintillators. This unique method provides valuable data used in the development of advanced ionizing radiation detectors for biomedical applications. We are currently addressing the following topics:

• Development of a laboratory SXR microscope
• Imaging of living cellular structures using XUV
• Characterization of materials for scintillation detectors 

Research topics

We are working on several research topics in the lab:

Development of the laboratory SXR microscope

The group has developed a compact transmission table-top SXR microscope operating in the “water window” region based on a Z-pinch capillary discharge plasma source, operating at a wavelength of 2.88 nm. The microscope can take images of the sample at 190× and 400× magnification with a spatial resolution of 75 nm. This resolution is a complementary imaging technique between conventional light microscopes and transmission electron microscopes. The microscope allows imaging of relatively thick samples (up to 10 um) and does not require fluorescent markers or other labeling and does not affect the morphology of the sample.

Imaging of living cellular structures using XUV

SXR microscopes are very suitable for imaging small biological samples. In the water window region (wavelengths of 2.3 to 4.4 nm) there is a large difference between the transmission of proteins and water. Almost all of the SXR radiation is absorbed by proteins, water remains for this radiation relatively transparent. This difference in transmission allows imaging of small living structures in aqueous environments with natural contrast.

Characterization of materials for scintillation detectors

Together with the Institute of Physics of the Czech Academy of Sciences and CRYTUR, spol. s r. o. we help to develop new products: fast single crystals and ionizing radiation detectors for harsh operating conditions. These are materials and detectors for applications with high radiation levels, such as monitoring industrial equipment using charged particle beams and interactions in laser and particle experiments. For these applications, the requirement is to use a scintillator with a fast scintillation response on the order of tens of nanoseconds. At FBME we use SXR as an excitation source to study the scintillation of materials.

Laboratory equipment

The key equipment of the laboratory includes two XUV (extreme ultraviolet) radiation sources based on 1) laser plasma, and 2) discharge plasma, a vacuum system with computer-controlled motors, a UV-VIS-NIR spectrophotometer with an integrating sphere, a nitrogen cryostat for optical measurements, an optical table and a nitrogen laser. The laboratory is mainly used for experimental activities of undergraduate, graduate and PhD students, but advanced laboratory tasks are also performed here.

Laboratory soft X-ray sources (SXR)

• Laser-generated plasma (incoherent)
• Laser for plasma generation:  Q-switched Nd:YAG 1064 nm, 800 mJ, 7 ns
• SXR wavelength range approx. 1-20 nm (depending on the target gas)
• SXR pulse length ~5 ns (Argon target)
• Photon flux ~1.7× 1013 photons per pulse/sr (Argon target)
• Various electrical, mechanical, and optical feedthroughs

• Discharge-generated plasma
• Wavelength 2.88 nm (430 eV; monochromatic)
• Pulse length SXR~ 20 – 50 ns
• Photon flux ~5.5 × 1013 photons per pulse/sr
• Spacious vacuum chamber with computer-controlled positioning system
• Various electrical, mechanical, and optical feedthroughs

Carl Zeiss ConfoCor2

• Fluorescence correlation spectroscopy
• Custom-upgrade TCSPC
• Fiber Ar laser for continuous wave (458, 488, 514 nm)
• Custom-upgrade for time resolved FCS
• PicoQuant TimeHarp 200 TCSPC
• PicoQuant PDL 800-B picosecond pulsed diode laser driver
• Pulsed laser heads 470 and 640 nm

Shimadzu UV-3600

• UV-VIS-NIR absorption spectrophotometer
• Thermoelectric temperature controller
• Detection range 185 - 3300 nm
• Double-beam
• Double monochromator with double grating, very low scattered light level, three detectors: photomultiplier (UV-VIS), InGaAs and PbS (near IR)

Publications, collaborations and projects

Selected publications

1. Wen X., Prusa P., Vladimir L., Parkman T., Vanecek V., Panek D., Nikl M., Cheng S., Wang Q., Ren G., Wu Y. (2023). Near-Infrared Emitting of Zero-Dimensional Europium(II) Halide Scintillators: Energy Transfer Engineering via Sm2+ Doping. ACS Applied Electronic Materials, 5 (6), 3507 - 3514.
2. Pokorný M., Babin V., Beitlerová A., Jurek K., Polák J., Houžvička J., Pánek D., Parkman T., Vaněček V., Nikl M. (2021). Gd-admixed (Lu,Gd)AlO3 single crystals: breakthrough in heavy perovskite scintillators. NPG Asia Materials, 13 (1), art. no. 66.
3. Parkman, T., Nevrkla, M., Jančárek, A., Turňová, J., Pánek, D., & Vrbová, M. (2020). Table-Top Water-Window Microscope Using a Capillary Discharge Plasma Source with Spatial Resolution 75 nm. Applied Sciences, 10 (18), 6373. 
4. Rubešová K., Havlíček J., Jakeš V., Nádherný L., Cajzl J., Pánek D., Parkman T., Beitlerova A., Kučerková R., Hájek F., Nikl M. (2019). Heavily Ce3+-doped Y3Al5O12 thin films deposited by a polymer sol-gel method for fast scintillation detectors. CrystEngComm, 21 (34), 5115 - 5123.
5. Pejchal J., Barta J., Babin V., Beitlerova A., Prusa P., Kucerkova R., Panek D., Parkman T., Guguschev C., Havlak L., Zemenova P., Kamada K., Yoshikawa A. (2018). Influence of Mg-codoping, non-stoichiometry and Ga-admixture on LuAG:Ce scintillation properties. Optical Materials, 86, 213-232.
6. Prusa, P., Kučera, M., Babin, V., Bruza, P., Parkman, T., Panek, D., ... & Pokorny, M. (2018). Tailoring and optimization of LuAG:Ce epitaxial film scintillation properties by Mg co-doping. Crystal Growth & Design, 18 (9), 4998-5007.
7. Torrisi A., Wachulak P., Wegrzynski Ł., Fok T., Bartnik A., Parkman T., Vondrová Š., Turňová J., Jankiewicz B.J., Bartosewicz B., Fiedorowicz H. (2017). A stand-alone compact EUV microscope based on gas-puff target source. Journal of Microscopy, 265: 251-260.
8. Vrba P., Vrbova M., Müller M., Mann K., Pánek D., Parkman T. (2017). Picosecond laser krypton plasma emission in water window spectral range. Physics of Plasmas, 24 (12)

Collaborations

The laboratory cooperates with top workplaces in the Czech Republic and abroad. Our domestic collaborators include the Institute of Physics of the CAS, the Faculty of Nuclear and Physical Engineering of the CTU. We cooperate with scientists at Brown University, USA, Military University of Technology, Poland, and Institut für Nanophotonik Göttingen (formerly Laser-Laboratorium Göttingen), Germany.

Projects

The laboratory is or has been involved in the following projects:

• TAČR: Fast detectors for harsh operating conditions, (2020 - 2023)
• GAČR: Fast thin-film scintillators for high-resolution 2D imaging, (2016 - 2018).
• GAČR: Pulsed soft X-ray source for biomedical applications,  (2012 - 2014)
• MŠMT: Development of the BIO-OPT-XUV research team at FBMI CTU, (2011 - 2014)

Contact

• Location of the laboratory    Room Cs-103, KOKOS building, FBME, Kladno
• Head of laboratory                Ing. Tomáš Parkman, Ph.D.
• E-mail                                    tomas.parkman@fbmi.cvut.cz

Department of Health Care Disciplines and Population Protection

Sportovců 2311, Kladno, 272 01

Who are we?

We are a team established in the Department of Health Care and Population Protection, focusing on highly topical research issues in the field of crisis management, public protection, chemical safety and emergency health care. We benefit from the fact that the department has brought together experts in emergency and disaster medicine with experts from the security forces with experience in crisis management, population protection, CBRN agents and security management in both theoretical and practical areas.

What research we do

Chemical, biological, radiological and nuclear substances

• Development of new bio dosimetry techniques with subsequent application in clinical practice
• Research of chemical reactions and development of technologies for field and laboratory analysis of chemical warfare agents and other safety-relevant toxic compounds, with an emphasis on testing them under near-realistic conditions
• Study and development of decontamination agents for application to individual hazardous chemical and radioactive agents and determination of the most effective technological procedures for decontamination in improvised conditions
• Study of firefighters´ exposure to combustion products and their effect on human health
• Development of the TOXALS methodology, of which personal protection, triage and decontamination are an integral part
• The problem of possible importation of highly dangerous diseases and their identification
• Issues of the effectiveness of air conditioning filters in terms of capturing infectious agents
• Software modelling of the dispersion of hazardous chemicals and mixtures in realistic environment

Study of pathophysiological processes in stressful situations and their prevention

• Study of the possibilities of increasing safety and operability of the IRS units by using personal surveillance systems. 
• Study of adaptation of the organism to cold and heat and possibilities of protection.
• Wound healing in extreme conditions and development of appropriate technologies.

Population protection and crisis management

• Innovation of principles, procedures, forces and means for warning, evacuation, emergency survival and individual protection of the population.
• Use of interactive virtual reality methods to support training of emergency services and security forces.
• Detection of radicalisation in the context of population protection and soft targets from violent incidents.
• Assessing the preparedness of an area against the threat of a large-scale power outage.
• Crisis management of hospital premises security processes and crisis planning in selected entities of the health sector.
• Elaboration of the principles of healthcare critical infrastructure and their application in relevant plans and methodological procedures.
• The psychological consequences of the impact of crisis situations on particular population groups.

What is it for

A significant danger to the population is industrialisation, the construction of mammoth industrial centres, where the failure of technology or the human factor can have disastrous consequences for the population, and not only for the population of the region. Other threats to the population stem mainly from the chemical industry, mass transport accidents, the occurrence of severe weather phenomena, interruptions in the supply of essential commodities and electricity, or threats to soft targets. The rapid movement of populations between continents with completely different epidemiological situations can lead to health threats to hosting populations, including pandemics. Large-scale consequences of natural disasters, disintegration of the energy network or mass attacks on information sources cannot be ruled out. Only an appropriately educated expert can handle these crisis situations rationally and effectively.

With whom we cooperate

• State Institute of Nuclear, Chemical and Biological Protection, v.v.i
• State Institute of Radiation Protection, v.v.i.
• Ministry of the Interior - General Directorate of the Fire Fighting Service of the Czech Republic
• Institute of Population Protection
• Fire Brigade of the Central Bohemian Region
• Fire Brigade of the Karlovy Vary Region
• Medical Rescue Service of the Central Bohemia Region
• Medical Rescue Service of the Karlovy Vary Region
• Expert Institute of Health and Safety, z.ú.
• University of Defence
• State Material Reserves Administration
• Refugee Facilities Administration of the Ministry of the Interior
• Army of the Czech Republic - 15th Engineer Regiment Bechyně
• Army of the Czech Republic - 31st Regiment of Radiation, Chemical and Biological Protection
• Police Presidium of the Czech Republic
• Regional Police Directorate of the Central Bohemian Region
• General Inspectorate of Security Forces
• Supreme State Prosecutor's Office
• National Drug Control Centre SKPV
• Customs Administration
• Probation and Mediation Service of the Czech Republic
• ORITEST spol. s r.o.
• SECURU s.r.o.
• T-SOFT a.s.
• XR Institute s.r.o.

Addressed projects

• VG 20102013048 Výzkum metod kontroly vody kontaminované toxickými látkami za mimořádných bezpečnostních situací, principal investigator ORITEST spol. s.r.o.
• NT 12156 Nanovlákenné nosiče s řízeným uvolňováním léčiv pro regeneraci osteochondrálních defektů.
• TA04010102 Systém pro monitorování a detekci – SYMOD, Co-recipient TESLA BLATNÁ, a.s., TTC TELEKOMUNIKACE s.r.o., Univerzita Pardubice, Západočeská univerzita v Plzni.
• TH01031098 Validace a verifikace modelu šíření a disperze těžkého plynu za specifických situací (DEGAS), Co-recipient T-SOFT a.s., ERGOWORK s.r.o., ÚJV Řež, a. s.
• FV20422 Vývoj nanovlákenných scaffoldů zajišťujících aplikaci celulárních produktů, včetně fyzikální stimulace účinku s účelem určení pro léčbu chronických ran, Co-recipient: Grade Medical s.r.o., Cellthera, s.r.o.
• VI20192022117 Detekce radikalizace v kontextu ochrany obyvatelstva a měkkých cílů před násilnými incidenty.
• FV40189 Inovativní cartridge pro manipulaci a aktivaci funkcionalizovaných nanovlákenných krytů ran ve zdravotnictví, Co-recipients: Grade Medical s.r.o.
• VI20192022124 Zvýšení odolnosti regionu před hrozbou plošného výpadku el. energie s využitím nových technologií a postupů krizového řízení, Co-recipients: FEL ČVUT, Teplárna Kladno s.r.o., SECURU s.r.o., PREdistribuce, a.s.
• VI20152020019 Výzkum kritických informačních struktur státu se zaměřením na jednotný systém varování a informování obyvatelstva, Co-recipient: Colsys s.r.o., Institut ochrany obyvatelstva.
• VG20102015002 Osobní bezpečnostní dohledový systém pro podporu výcviku a zásahu jednotek IZS.
• TL05000480 Zvýšení kvality života v domovech pro seniory v období nouzového stavu, Co-recipient: Vysoká škola AMBIS.
• VK01020196 Inovativní systém využití virtuální reality a simulovaných modelových případů bezpečnostního charakteru usnadňující výcvik a reakci příslušníků policie v rizikových situacích, Co-recipient: XR Institute s.r.o.
• VK01010037 Metodika rychlé bezkontaktní a nedestruktivní detekce zplodin výstřelu,  Co-recipients: Západočeská univerzita v Plzni / Nové technologie - výzkumné centrum, Ministerstvo vnitra / Policie ČR Kriminalistický ústav.
• VK01020078 Chytrý systém pro nositelné ochranné pomůcky umožňující dohled a plánování policejních a armádních zásahů, Co-recipients: Univerzita obrany - Fakulta vojenských technologií Brno, Vysoká škola chemicko-technologická v Praze / Fakulta chemické technologie, Západočeská univerzita v Plzni / Fakulta elektrotechnická.
• VJ02010037 Monitorování polohy příslušníků složek IZS i během zásahu v rozsáhlých budovách s využitím prvků umělé inteligence, Co-recipient: Vysoká škola báňská - Technická univerzita Ostrava / Fakulta elektrotechniky a informatiky.

Department of Biomedical Technology, nám. Sítná 3105, Kladno, 272 01

prof. Ing. Karel Roubík, Ph.D.

Who we are?

We are a team of experts interested in mechanical ventilation and various aspects of technology for anesthesia, resuscitation and critical care medicine. Selected results of our work can be found at www.ventilation.cz.

Research team:

prof. Ing. Karel Roubík, Ph.D.
doc. Ing. Martin Rožánek, Ph.D.
doc. Ing. Petr Kudrna, Ph.D.
MUDr. Lenka Horáková, Ph.D., DESA
Ing. Jakub Ráfl, Ph.D.
Ing. Václav Ort, Ph.D.
Ing. Leoš Tejkl, Ph.D.
Ing. Veronika Ráfl-Huttová, Ph.D.
Ing. Kristýna Koldová, Ph.D.
Ing. Šimon Walzel
Ing. Ladislav Bís
M.Sc. Thomas E Bachman
MUDr. Michal Soták, Ph.D.
MUDr. Aleš Rára, Ph.D.
MUDr. Martin Muller, Ph.D.
MUDr. David Novotný
MUDr. Jan Páleník
and students of bachelor's and master's degree programs

Ongoing grants:

• SGS23/198/OHK4/3T/17 (Increase of Safety and diagnostic value of medical equipment in respiratory care)
• SGS25/110/OHK4/2T/17 (Wearable and wireless devices for continuous vital signs monitoring)
• KH-2023-045-DO-12181 (Support for Education and Promotion of Biomedical Engineering in Cambodia)

Cooperation:

1. Central Military Hospital – Military University Hospital Prague
2. Institute of Physiology, Faculty of Science, Charles University 
3. Department of Anesthesiology and Resuscitation, University Hospital Královské Vinohrady, Third Faculty of Medicine, Charles University
4. Department of Anesthesiology and Resuscitation, Thomayer University Hospital, Charles University
5. University of Health Sciences, Cambodia
6. Institute of Technology of Cambodia
7. Czech University of Life Sciences Prague (CZU)
8.  Inspamed (Institute of Sleep Medicine)
9. MICo Medical s.r.o.
10. Army of the Czech Republic
11. Faculty of Physical Education and Sport, Charles University (FTVS UK)

Research Projects:

Breathing experiments into the simulated avalanche snow

Avalanche burial represents one of the greatest risks associated with winter activities in the mountains. Asphyxiation occurs as a result of blocked airways or due to severe hypoxia and hypercapnia caused by rebreathing previously exhaled air. The physical properties of snow and the diffusion of gases play a crucial role in determining the survival time of individuals trapped in avalanche snow. Understanding how snow affects gas exchange can help improve survival strategies and the development of safety equipment, ultimately increasing the chances of survival in such extreme situations.

Selected publications (2020-2025):

• Roubik, K., Sykora, K., Sieger, L., Ort, V., Horakova, L., & Walzel, S. (2022). Perlite is a suitable model material for experiments investigating breathing in high-density snow. Scientific Reports, 12(1), 2070.
• Walzel, S., Rozanek, M., & Roubik, K. (2023). Perlite has similar diffusion properties for oxygen and carbon dioxide to snow: Implications for avalanche safety equipment testing and breathing studies. Applied Sciences, 13(23), 12569.
• Horakova, L., & Roubik, K. (2022). Pulse oximeter performance during rapid desaturation. Sensors, 22(11), 4236.
• Roubik, K., Walzel, S., Horakova, L., Refalo, A., Sykora, K., Ort, V., & Sieger, L. (2020). Materials suitable to simulate snow during breathing experiments for avalanche survival research. Clinician and Technology, 50(1), 32-39.

Design of CoroVent ventilator

The CoroVent ventilator was developed in response to the critical shortage of ventilators during the COVID-19 pandemic. This project was led by a team of experts from the Faculty of Biomedical Engineering at the Czech Technical University in Prague, in collaboration with the COVID19CZ platform. The goal was to create an affordable, functional, and easily manufacturable ventilator that could be used in crisis situations, such as pandemics or natural disasters. Although the development focused on simplicity of construction and ease of maintenance, safety was also a key consideration. The unique principle of the ventilator lies in the simplicity of creating inspiratory flow and gas mixing, which allows for easy customization and extension of functions as needed. Additionally, the ventilator is equipped with systems for monitoring pressure and flow in the patient’s airways and a variety of alarms, ensuring maximum patient safety and ease of operation for the staff. After receiving emergency use authorization from the FDA in the summer of 2020, CoroVent also received an exemption for use in Czech hospitals. Since October 2020, it has been distributed to 27 hospitals, helping to increase the capacity of intensive care units, with several cases of clinical use.

Selected publications (2022-2025):

• Roubik, K., Ort, V., Horakova, L., & Walzel, S. (2023). Novel design of inspiratory flow generation and gas mixing for critical care ventilators suitable for rapid production and mass casualty incidents. Scientific Reports, 13(1), 7153.
• Bís, L., & Roubík, K. (2022). Design and performance of a flow sensor CoroQuant used with emergency lung ventilator CoroVent during COVID-19 pandemic. Measurement: Sensors, 22, 100383.
• Roubik, K., Skola, J., Horakova, L., Ort, V., & Walzel, S. (2021, November). First Clinical Use of Rapidly Designed and Manufactured Mechanical Lung Ventilator CoroVent for COVID-19 Patients. In 2021 International Conference on e-Health and Bioengineering (EHB) (pp. 1-4). IEEE.
• Roubík, K., & Ort, V. (2022). Způsob provádění umělé plicní ventilace a zařízení k provádění tohoto způsobu [Method of performing artificial lung ventilation and a device for performing this method] (Patent No. 309212). Czech Technical University in Prague. Industrial Property Office of the Czech Republic. https://isdv.upv.cz

Wearable and Wireless Devices for Continuous Monitoring of Vital Signs

Wearable and wireless devices represent an accessible and non-invasive tool for continuous monitoring of cardiovascular health. For example, the reliability of heart rate measurements and, more recently, the accuracy of blood oxygen saturation measurements using smartwatches has already been verified, while the accuracy and reliability of blood pressure and ECG measurements remain subjects of ongoing research. The main benefit lies in the potential for early diagnosis of heart conditions (such as atrial fibrillation and hypertension). The development of these technologies also presents challenges in the areas of certification and standardization for clinical practice. The project primarily aims at independently verifying the reliability of smartwatches through prospective studies, including both healthy volunteers and patients with various health issues.

Selected publications (2022-2025):

• Rafl, J., Bachman, T. E., Rafl-Huttova, V., Walzel, S., & Rozanek, M. (2022). Commercial smartwatch with pulse oximeter detects short-time hypoxemia as well as standard medical-grade device: Validation study. Digital health, 8, 20552076221132127.
• Walzel, S., Mikus, R., Rafl-Huttova, V., Rozanek, M., Bachman, T. E., & Rafl, J. (2023). Evaluation of Leading Smartwatches for the Detection of Hypoxemia: Comparison to Reference Oximeter. Sensors, 23(22), 9164.

Use of Electrical Impedance Tomography for Cardiovascular and Respiratory System Monitoring

Electrical impedance tomography (EIT) is a non-invasive method primarily used for continuous monitoring of lung ventilation, but it also allows the detection of changes in the distribution of fluids in the body. The project focuses on improving the interpretation of EIT data and distinguishing between true physiological changes and artifacts caused by intravenous infusions. EIT enables the detection of changes in pulmonary perfusion, which can be crucial for monitoring the effectiveness of fluid therapy, especially in critically ill patients. Experiments on animal models have demonstrated that EIT can detect changes in bioimpedance caused by intravenous fluid administration, opening up possibilities for personalized management of infusion therapy. The goal of the research is to verify the reliability of EIT for assessing the body’s response to fluid intake and identifying potential risk factors, such as edema or hypoperfusion. This approach could allow physicians to quickly and non-invasively assess whether a patient is benefiting from fluid administration or whether infusion therapy needs to be slowed or stopped, which would have a direct impact on improving patient outcomes in intensive care.

Selected publications (2019-2025):

• Rara, A., Roubik, K., Tyll, T. Effects of pleural effusion drainage in the mechanically ventilated patient as monitored by electrical impedance tomography and end-expiratory lung volume: A pilot study. Journal of Critical Care, 59, October 2020, pp. 76-80.
• Ort V, Roubik K. Electrical Impedance Tomography Can Be Used to Quantify Lung Hyperinflation during HFOV: The Pilot Study in Pigs. Diagnostics. 2022; 12(9):2081.
• Koldova, K.; Rara, A.; Muller, M.; Tyll, T.; Roubik, K. Cranial Electrode Belt Position Improves Diagnostic Possibilities of Electrical Impedance Tomography during Laparoscopic Surgery with Capnoperitoneum. Sensors 2023, 23, 8644.
• Sobota V, Müller M, Roubík K. Intravenous administration of normal saline may be misinterpreted as a change of end-expiratory lung volume when using electrical impedance tomography. Scientific Reports. 2019 Apr 8;9(1):5775.

Physiological Monitoring and Intervention in Mechanical Ventilation

The project focuses on improving the understanding of interactions between the respiratory system and mechanical ventilation in order to enhance its effectiveness and safety. The work includes the development and validation of methods for monitoring mechanical and physiological parameters during ventilation, including the use of advanced technologies and physical models. The research involves both experimental and clinical studies—using in vitro modeling, animal experiments, and clinical data—aimed at optimizing ventilator settings and minimizing the adverse effects of prolonged mechanical support, such as diaphragm weakening. The project provides new insights that may contribute to reducing the duration of ventilation, accelerating patient recovery, and improving overall outcomes in intensive care.

Selected publications (2021-2025):

• Soták, M., Roubík, K., Henlín, T. et al. Phrenic nerve stimulation prevents diaphragm atrophy in patients with respiratory failure on mechanical ventilation. BMC Pulm Med 21, 314 (2021).
• Matejka, J.; Rozanek, M.; Rafl, J.; Kudrna, P.; Roubik, K. In Vitro Estimation of Relative Compliance during High-Frequency Oscillatory Ventilation. Appl. Sci. 2021, 11, 899.
• Roubík, K., Ráfl, J., Rožánek, M. et al. Tidal volume significantly affects oxygenation in healthy pigs during high-frequency oscillatory ventilation compared to conventional ventilation. BioMed Eng OnLine 21, 14 (2022).
• Soták, M., Tyll, T., & Roubík, K.: Temporary phrenic nerve stimulated patients: What is the role of ultrasound examination? Artificial Organs, 47(3), 464-469 (2023).
• Walzel, S., & Roubik, K. (2024). Effect of tissue viscoelasticity on delivered mechanical power in a physical respiratory system model: distinguishing between airway and tissue resistance. Biomedical Physics & Engineering Express, 11(1), 015026.

Scientific teams

Department of Information and Communication Technologies in Medicine, Studničkova 7/2028 Prague 2

Doc. Ing. Karel Hána, Ph.D

Team / project name

Rehabilitation process quantification

Who we are?

Ondřej Antoš, Karel Hána, Markéta Janatová, Jaroslav Jeřábek, Jan Kašpar, Vojtěch Malina, Jan Mužík, Kateřina Pilátová, Pavel Smrčka, Luisa Šedivcová

What research are we doing?

We are interested in monitoring and quantifying the process of rehabilitation care of patients with focal brain injury, using elements of virtual reality, mobile applications and telemedicine systems. We are focused on technologies for the rehabilitation of patients with balance disorders or other related difficulties.

What is it for?

The goal of all rehabilitation procedures is to improve the patient's functional abilities. A number of rehabilitation procedures lead to this goal, which work with the patient in the given environment of the rehabilitation ambulance, or with the help of distant therapy, when the patient rehabilitates in the home environment. The aim of all procedures is to improve the quality of life of patients. Rehabilitation, especially in the initial stages, represents a relatively large burden for a target person, which can significantly affect the functionality of the cardiovascular system and when a certain limit of exercise tolerance is exceeded, rehabilitation may slow down or worsen. That is why we deal with technologies, telemedicine systems and procedures that support the process of distant care.

What exactly are we working on

The issues we are dealing with can be divided into three main areas:

1. Monitoring of a rehabilitating patient in terms of his physical activities and the response of the cardiovascular systém

This part takes place in close cooperation with the Department of Rehabilitation Medicine, First Faculty of Medicine, Charles University and the General Hospital, which admits patients after brain damage to the program of the so-called day hospital, where they are rehabilitated for 3 weeks. At present, it is not objectified how much time they spend exercising, how they burden the affected and healthy limbs and how much stress the cardiovascular system occurs in rehabilitated patients.

The goal is:

• to obtain an overview of the physical activities of patients rehabilitated within the day hospital, on the basis of this overview it will be possible to optimize the daily regime of patients
• using HRV analysis to determine the degree of stress and cardiovascular load in general in individual phases of rehabilitation
• The analysis of the movement of the affected limb, respectively the improvement of its use during rehabilitation, should provide essential information. Improving movement, ie the gradual involvement of the affected limb in physical activities, is proof of the success of the rehabilitation. Recording of gradual improvement of limb movement resp. limbs would be a measurable and objective record of a better patient. Based on these data, it would then be possible to optimize the rehabilitation procedure.

Based on this information and the experience gained, another goal should be fulfilled in the next phase - monitoring the success of the rehabilitation process carried out in the home environment. From a purely technical point of view, this would be the design and construction of a device enabling the above-mentioned measurements to be carried out during rehabilitation carried out at home. On the one hand, this technical equipment would bring very valuable information about physical activities, time and duration of exercise, the quantitative parameters of which it would be able to continuously monitor.

2. Use of virtual reality for rehabilitation of patients with balance disorders

Balance is ensured in the organism by the interaction of three sensory inputs: visual, vestibular and proprioceptive. Therefore, imbalances can occur when individual systems or centers fail, which fold for their mutual coordination. The standard procedure in the rehabilitation of balance disorders is the use of biological feedback, which uses a stabilometric platform on which the patient stands and monitors the movement of his center of gravity on the screen.

The department participates in research and development of new medical devices in the field of rehabilitation. The developed medical devices include, for example, a patient device consisting of a stabilometric platform, a wrist pedometer and a tablet with a program containing therapeutic games.

Telemedicine expansion can allow remote configuration of a therapeutic exercise plan and remote recording of the course and results of therapy. The therapy also includes a web environment for the management of distance therapy and evaluation of health improvement. Developed medical devices are intended for therapy and comparative evaluation of patients with motor and cognitive impairments due to congenital or acquired brain damage, in geriatric patients, and other patient groups with the need to improve stability and support balance and cognitive training.

The results of the research are used by patients of various rehabilitation institutions after injuries or surgical procedures of the musculoskeletal and nervous system. Specialists, general practitioners, physiotherapists or other specialists in hospitals, nursing homes or spas.
Users can be clinics, dispensaries and their clients undergoing rehabilitation for various reasons, where nursing staff will appreciate technological advances in care, electronic procedures and support for home therapy provided remotely. It will improve the quality of care and increase the quality of life of patients suffering from a variety of disabilities, including increasing the comfort of life for their families.

3. Telerehabilitation and patient studies

The university workplace is a professional guarantor and implementer of scientific and technological activities that provide tools, methods and a support system for the implementation of user / patient studies in the field of rehabilitation and telemedicine. The department provides patient research in the form of identification of user needs, determination of requirements for therapeutic / exercise plans and carries out the actual implementation of the pilot deployment of telerehabilitation technology, including the validation of follow-up processes.

Who funds our research

• Research projects
• Resources from specific research - cooperation with students
• Joint workplace of biomedical engineering FBMI and 1.LF UK in Prague FBMI CTU in Prague
• 1st Faculty of Medicine, Charles University in Prague
• Thanks to the support of CleverTech s.r.o., spin-off company FBMI and 1.LF UK in Prague.

Who we work with

• Department of Rehabilitation Medicine, First Faculty of Medicine, Charles University and General Hospital
• Department of Neurology, First Faculty of Medicine, Charles University and General Hospital in Prague
• Department of Neurology for Adults, 2nd Faculty of Medicine and Motol University Hospital
• CleverTech s.r.o., spin-off company FBMI CTU and 1.LF UK in Prague
• HomeBalance s.r.o., spin-off company 1.LF UK in Prague
• ARTAK, Czech Association of Robotics Telemedicine and Cybernetics
• Rehabilitation Institute Kladruby

Selected publications

1. Hana, K., Kaspar, J., Kucera, L., Muzik, J., Smrcka, P., Vesely, T., Viteznik, M.: Method of wireless connection of an intelligent house with a rescue system patrol and a system for its implementation. Patent 308531, 16.9.2020, Industrial Property Office in Czech Republic, 2020
2. Hana, K., Kaspar, J., Kucera, L., Muzik, J., Smrcka, P., Vesely, T., Viteznik, M., Kliment, R.: Monitoring and transport system especially for the transport of infectious patients. Patent 307932, 17.7.2019, Industrial Property Office in Czech Republic, 2019
3. Hana, K., Kaspar, J., Kucera L., Muzik, J., Smrcka, P., Vesely, T., Viteznik, M.: Surveillance equipment for monitoring persons, especially in difficult conditions, and a system for placing sensors on the human body. Patent 307930, 17.7.2019, Industrial Property Office in Czech Republic, 2019
4. Hlavinka, P., Sebelka, Z., Hana, K., Kaspar, J., Muzik, J., Smrcka, P.: Method of three-stage communication of the notification center and the end element of the warning. Patent 307931, 17.7.2019, Industrial Property Office in Czech Republic, 2019
5. Muzik, J., Viteznik, M., Hana, K., Smrcka, P., Kaspar, J., Funda, T., Kudlicka, J., Mlcek, M., Streda, L.: Low energy defibrillation equipment. Patent 306991, 20.9.2017, Industrial Property Office in Czech Republic, 2017
6. Kaspar, J.; Hon, Z.; Janatova, M.; Smrcka, P.; Viteznik, M.; Hana, K.; Vesely T.; Muzik, J.: Biotelemetric system to support monitoring of the psychophysiological state of man. Patent 306895, 19.7.2017, Industrial Property Office in Czech Republic, 2017
7. Janatova, M.; Ticha, M.; Hana, K.; Svestkova, O.: Use of force platform and visual feedback in home-based therapy of patients with brain injury. In: Brain Injury, vol. 31, Issue 6-7, 2017, ISSN: 0269-9052, IF1,97 (2016)

doc. Ing. Patrik Kutilek, M.Sc., Ph.D.
E-mail: kutilek@fbmi.cvut.cz

We are a multidisciplinary team dedicated primarily to, but not limited to, research in biomechanics and assistive technologies. Above all, we focus on the study of kinematics and dynamics of motion and physiological data, and the design of electronic and mechatronics systems designed to measure and evaluate the movement and physiological data of humans and animals. We are also engaged in ergonomic and the development and testing of mechanical elements of medical devices, especially assistive devices. We take advantage of the fact that specialists with knowledge of robotics, informatics and medicine, with experience in the fields of simulations, measurement, processing and analysis of mechanical, physical data, met at the FBME CTU.

Our research

We develop methods and systems for recording and evaluating motion and physiological data, developing methods and systems for recording and evaluating force and moment effects, designing mechanical parts of assistive devices, developing medical software for assistive devices.

Team members

doc. Ing. Patrik Kutílek, M.Sc., Ph.D.; Ing. Lýdie Leová, Ing. et Ing. Jan Hejda, Pd.D.; Ing. Petr Volf; Ing. Marek Sokol, Bc. Jan Tonner, Ing. Veronika Kotolová, Bc. Blanka Sliacká, etc.

Publications

1. Sokol M., Hejda J., Volf P., Kutílek P. Multimodal Emotion Recognition Through a Hybrid CNN-GCN Model Integrating Neuroimaging and Physiological Data (2025) IFMBE Proceedings, 118 IFMBE, pp. 399 - 405
2. Adolf J., Segal Y., Turna M., Nováková T., Doležal J., Kutílek P., Hejda J., Hadar O., Lhotská L. Evaluation of functional tests performance using a camera-based and machine learning approach (2023) PLoS ONE, 18 (11 NOVEMBER), art. no. e0288279
3. Sokol M., Volf P., Hejda J., Leová L., Hýbl J., Schmirler M., Suchý J., Procházka R., Charvát M., Seitlová K., Dolejš M., Schneider J., Kutílek P. DIANA: An underwater analog space mission (2025) Acta Astronautica, 226, pp. 349 - 360
4. Kutilek P., Karavaev A., Hejda J., Krivanek V., Hyb J., Hájková S., Volf P. Control System and User Interface of Cooling Module for Braces
   (2021) IFMBE Proceedings, 80, pp. 286 - 293
5. Hejda J., Kutilek P., Volf P., Sokol M., Leova L., Tonner J., Hejskova M., Kotolova V., Rozloznik M., Sugiarto T., Lin Y.-J., Huang K.-L., Hsu W.-C. Wearable System for Monitoring the Physical Conditions in Isolated, Confined and Extreme Environments (2024) Proceedings of the 2024 21st International Conference on Mechatronics - Mechatronika, ME 2024,
6. Hejda J., Volf P., Sokol M., Leová L., Kutílek P. Spinal Curvature Estimation Using Low-Cost Portable Sensors
   (2025) IFMBE Proceedings, 118 IFMBE, pp. 32 - 37
7. Volf P., Hsu W.-C., Hejda J., Lin Y.-J., Kutilek P., Sugiarto T., Sokol M., Leova L., Tsai H.-L., Chang S.-Y., Tang L.-X. Use of Nonlinear Analysis Methods for Visual Evaluation and Graphical Representation of Bilateral Jump Landing Tasks (2023) 2023 IEEE 5th Eurasia Conference on Biomedical Engineering, Healthcare and Sustainability, ECBIOS 2023, pp. 75 - 78, Cited 0 times.
8. Rozloznik M., Schneider J., Hejda J., Kutilek P., Volf P., Sokol M., Hsu W.C., Huang K.L., Lin Y.J., Sugiarto T., Tang L.X., Lin Y.C. Tool for real-time monitoring and analysis of the exercise in the ICE environment (2024) Proceedings of the International Astronautical Congress, IAC, 2, pp. 798 - 801, Cited 0 times.
9. Volf P., Hejda J., Sokol M., Leová L., Lin Y.-J., Sugiarto T., Kutílek P. RGB-D Motion Capture System for Monitoring Health Conditions of Employees in Physically Demanding Professions (2025) IFMBE Proceedings, 118 IFMBE, pp. 38 - 44
10. Žarković, D., Šorfová, M., Tufano, J.J., Kutílek, P., Vítečková, S., Ravnik, D., Groleger-Sršen, K., Cikajlo, I., Otáhal, J.; Gait changes following robot-assisted gait training in children with cerebral palsy (2021) Physiological research, 70 (S3), pp. S397-S408.
11. Leova, L., Cubanova, S., Kutilek, P., Volf, P., Hejda, J., Hybl, J., Stastny, P., Vagner, M., Krivanek, V. Current State and Design Recommendations of Exoskeletons of Lower Limbs in Military Applications (2022) Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics), 13207 LNCS, pp. 452-463. 
12. Mitriková, A., Hejda, J., Volf, P., Bačíková, M., Oberman, Č., Rusnáková, K., Braunová, M., Kutílek, P. Pilot Study of Application of a Hybrid Transportable System for Postural Stability Measurement in Military Professions (2021) IFMBE Proceedings, 80, pp. 968-975. 
13. Kutilek, P., Hejda, J., Volf, P., Krivanek, V., Cicmanec, L., Hana, K., Smrcka, P., Fajnerova, I.Evaluation of Psychological Load of Air Defense Members by Physiological Data Monitoring Compared to the Questionnaire Evaluation Method (2020) Proceedings of the 2020 19th International Conference on Mechatronics - Mechatronika, ME 2020, art. no. 9286626,
14. Volf, P., Bernaskova, K., Hejda, J., Francova, A., Kutilek, P., Hybl, J., Hourova, M. Car Simulator for Selection and Screening of Patients after Brain Injury (2020) Proceedings of the 2020 19th International Conference on Mechatronics - Mechatronika, ME 2020, art. no. 9286600,
15. Volf, P., Kutilek, P., Hejda, J., Viteckova, S., Smrcka, P., Hana, K., Svoboda, Z., Krivanek, V. Methods for kinematic analysis of human movement in military applications: A review of current and prospective methods (2020) Lekar a Technika, 49 (4), pp. 125-135. 
16. Hejda, J., Volf, P., Bačíková, M., Bar, N., Oberman, C., Rusnáková, K., Braunová, M., Kutílek, P. Design of a hybrid portable system for measuring the position of the spine, pelvis and center of gravity of the body (2020) IFMBE Proceedings, 76, pp. 622-633.
17. Hejda, J., Volf, P., Mejstřík, J., Hýbl, J., Tvrzník, A., Gerych, D., Michálek, T., Oberman, Č., Bolek, E., Kutílek, P. Design of device for measuring the load of cross-country ski poles (2020) IFMBE Proceedings, 76, pp. 640-649. 
18. Kliment, R., Smrčka, P., Hána, K., Schlenker, J., Socha, V., Socha, L., Kutílek, P.; Wearable modular telemetry system for the integrated rescue system operational use (2017) Journal of Sensors, 2017, art. no. 9034253.
19. Kutilek, P., Mares, J., Hybl, J., Socha, V., Schlenker, J., Stefek, A.; Myoelectric arm using artificial neural networks to reduce cognitive load of the user (2017) Neural Computing and Applications, 28 (2), pp. 419-427.
20. Svoboda, Z., Janura, M., Kutilek, P., Janurova, E.; Relationships between movements of the lower limb joints and the pelvis in open and closed kinematic chains during a gait cycle (2016) Journal of Human Kinetics, 50 (2), pp. 37-43.
21. Schlenker, J., Socha, V., Riedlbauchová, L., Nedělka, T., Schlenker, A., Potočková, V., Malá, Š., Kutílek, P.; Recurrence plot of heart rate variability signal in patients with vasovagal syncopes (2016) Biomedical Signal Processing and Control, 25, pp. 1-11.
22. Kutilek, P., Cakrt, O., Socha, V., Hana, K.; Volume of confidence ellipsoid: A technique for quantifying trunk sway during stance (2015) Biomedizinische Technik, 60 (2), pp. 171-176.
23. Hejda, J., Cakrt, O., Socha, V., Schlenker, J., Kutilek, P.;3-D trajectory of body sway angles: A technique for quantifying postural stability (2015) Biocybernetics and Biomedical Engineering, 35 (3), art. no. 72, pp. 185-191.
24. Kutilek, P., Socha, V., Viteckova, S., Svoboda, Z.; Quantification of gait asymmetry in patients with ankle foot orthoses based on hip-hip cyclograms (2014) Biocybernetics and Biomedical Engineering, 34 (1), pp. 46-52.
25. Bizovska, L., Svoboda, Z., Kutilek, P., Janura, M., Gaba, A., Kovacikova, Z.; Variability of centre of pressure movement during gait in young and middle-aged women (2014) Gait and Posture, 40 (3), pp. 399-402.
26. Viteckova, S., Kutilek, P., Jirina, M.; Wearable lower limb robotics: A review (2013) Biocybernetics and Biomedical Engineering, 33 (2), pp. 96-105.
27. Kutilek, P., Viteckova, S., Svoboda, Z., Smrcka, P.; Kinematic quantification of gait asymmetry in patients with peroneal nerve palsy based on bilateral cyclograms (2013) Journal of Musculoskeletal Neuronal Interactions, 13 (2), pp. 244-250.
28. Hejda, J., Kutilek, P., Hozman, J., Cerny, R.; Motion capture camera system for measurement of head and shoulders position (2012) Biomedizinische Technik, 57 (SUPPL. 1 TRACK-B), pp. 472-475.
29. Mikšovský, J., Kutílek, P., Lukeš, J., Tolde, Z., Remsa, J., Kocourek, T., Uherek, F., Jelínek, M.; Adhesion properties of DLC and TiO<inf>2</inf> thin films using scratch test methods (2011) Chemicke Listy, 105 (17).

Department of Information and Communication Technologies in Medicine
Studničkova 7/2028 Praha 2
Ing. Jan Mužík, Ph.D.

Who we are?

Multidiscplinary team consisting of experts from FBMI CTU, 1st and 2nd Faculty of Medicine and CIIRK CTU

Members:

Jan Mužík, Ann Holubová, Jan Brož, Dominik Fiala, Marek Doksanský, David Gillar, Tomáš Kučera, Tomáš Kuttler, Jan Kašpar, Pavel Smrčka, Karel Hána, Miroslav Mužný, Martina Vlasáková, Ondřej Pelák, Klára Bajerová, Patrícia Štefanová

What research do we do?

We focus mainly on the design of telemedicine systems used to monitor and support the treatment of chronically ill patients (esp. Patients with diabetes mellitus, hypertension and cardiovascular diseases), to support and motivate movement in hemiparetic patients and patients with mental illnesses, and last but not least prevention of associated diseases.
These systems include especially applications for smartphones, wearable electronics and mobile medical devices, smart scales, pressure gauges, etc. Emphasis is placed on wireless communication and automatic data collection.

Current projects and clinical studies:
Projects:

Diani telemedicine system (http://www.albertov.cz/projekty/diani/) for support of patients with diabetes

• Diani web application development (https://www.diani.cz/)
• development of a mobile application of the Diabetesdagboka diabetic diary
• Development of other android applications focused on self-management of diabetes and serious games
• Development of a telecommunications system for families with diabetic children
• Online monitoring and data collection in patients with DM1 and DM2
• collection and analysis of data from wearable electronics and medical devices (glucometers, CGM, insulin pumps, pressure gauges, scales, pedometers, etc.)

• Telemedicine system for patients with hypertension and cardiovascular disease (arrhythmia)
  • Online monitoring and data collection in patients with hypertension and cardiovascular disease
  • ollection and analysis of data from medical devices (pressure gauges with support for the detection of cardiac abnormalities) + support for the recording of other medical information
• SOMA: Physical Health Care Project and Training for Independent Living Abilities
  • Emed Telemedicine system for Bohnice Psychiatric Hospital
  • monitoring of physical activity in schizophrenic patients to support their health and prevent the development of associated diseases or suppress their development
  • connection of the system to NIS (Hippo)

Clinical studies:

• Faculty Hospital Motol:
  • Influence of telemedicine system on the quality of life of patients with type 1 diabetes mellitus
  • Influence of physical activity and other parameters on diabetes mellitus compensation - possibilities of telemonitoring and computer data processing within expert system
• Rehabilitation Center Kladruby:
  • Influence of the involvement of hemiparetic patients on pedometer accuracy and possibilities of their use for long-term monitoring

Results of our work:

• mobile application for smartphones Diabetesdagboka (in cooperation with NSE, Norway)
  • diabetic log for recording blood glucose values, injected insulin doses,carbohydrates consumed, and physical activity (https://play.google.com/store/apps/details?id=no.telemed.diabetesdiary)
• Diabetesdagboka app for smartwatches Pebble Now (in collaboration with NSE, Norway)
  • automatic transfer of records entered through the watch into the application
• Diani web application (https://www.diani.cz/)
  • collection, analysis and online monitoring of measured parameters in patients with DM
  • automatic synchronization of records from the Diabetesdagboka mobile app
  • displaying data from continuous glucose monitors, activity trackers, smart scales, pressure gauges, insulin pumps
• diabetic watch application for AndroidWear
  • automatic transmission and display of blood glucose values measured by personal meter
  • display data in the form of spiral graphs
  • a tool for identifying glycemic excursions at a certain stage of the day and increasing adherence to regular glycemic control
• SOMA web application
  • ollection and online monitoring of physical activity records in patients with mental illness

Who we cooperate with?

• Internal clinics of 2nd Medical Faculty UK and Faculty Hospital Motol
• Norwegian Centre for E-health Research, Tromso(https://ehealthresearch.no/en/)
• University of  Tromsø The Arctic University of Norway (https://en.uit.no/startsida)
• DiabetesLab
• University of Applied Sciences | Technikum Wien

Student projects

• Analysis of Dagboka application tracking and frequency tracking data used by DM1 patients
• Design and implementation of Dagboka application changes according to human typology using data from personality questionnaire and application using DM1 patients
• Interactive toys for children as a tool for barrier-free communication between a child with pre-school DM1 and a parent
• Design and implementation of glycemic watches using spiral graphs
• Relationship between heart rate and actual blood glucose levels during sleep and the possibility of using continuous heart rate monitoring as a tool for early detection of hypoglycemia
• Possibilities of using surveillance system and online monitoring of selected parameters in patients with chronic illness who live alone
• The complete list can be found on the Albertov website (http://www.albertov.cz/studentske-projekty/)

Photo gallery

Contacts:

Topics of interest

Fabrication and study of thin layers of materials for implantology, tissue engineering and medicine. We fabricate and study thin layers of biocompatible materials, namely:

• Hydroxyapatite for better osseointegration of implants (tooth, hip replacement)
• Diamond-like-carbon for better biocompatibility (minimization of immune response a friction) implants such as joint replacement, arterial stents, heart valves
• Titanium dioxide for photocatalytic and antibacterial applications for medical equipment (for example: urethral catheter)
• Silver for antibacterial applications on implants
• Organic and polymer materials (MAPLE technology) for sensors and tissue engineering
• Bioglass, zircon, doped biocompatible layers (Ag, Mo, Cr, Ti …), nanocrystalline and nanocomposite layers, atd.

The goal is to develop new types biocompatible thin layers for application in medicine and sensor fields.

Modification of implant surface

We modify the surface of implant materials via mechanical, laser, and plasma (O2, NH2, O3) treatment for better biocompatibility. Resulting surfaces are studied with emphasis on support or inhibition of different cell growth.

Interaction study of UV laser irradiation with material

Interaction process of laser irradiation with material (biological tissue) is studied with thermocamera, fast infrared detectors, optically and spectroscopically. Damage or modification of tissue is evaluated in cooperation with medical faculties.

Preparation of nanoparticles of metals and silicon

We synthetize nanoparticles via pulsed laser ablation in liquid for drug delivery systems and biomolecular tagging.

Workplace

Most of our work take place in the Laboratory of excimer laser in the building of Institute of Physics of the Czech Academy of Sciences (Na Slovance 1999/2, 182 21 Prague 8) and laboratories at the Department of Natural Sciences at FBME CTU (sq. Sítná 3105, 272 01 Kladno).

Laboratory of excimer laser belongs to the Institute of Physics of the Czech Academy of Sciences, Department of optical and biophysical systems and Department of Natural Sciences FBMI CTU participates in this department by loaning equipment, cooperation of persons and measuring and evaluating results in laboratories at FBMI.

Experimental equipment

• KrF a ArF excimer laser Compex 205F for layers fabrication via pulsed laser deposition
• Contact angle measurement system Krüss DS 100 for determination of wetting and surface energy
• Atomic Force MicroscopeSolver Next (NT- MDT) for measuring of topography, elastic properties, adhesion microhardness
• Fourier Transform InfraRed Spectrometer (Nicolet 6700) for chemical analysis of layers and materials (gases, liquids, solids)
• Rapid Thermal Annealing device - Solaris 75 (Surface Science Integration) for prepared layers modification (recrystallization)
• Profilometer Alphastep IQ (KIA Tencor) for thickness and roughness measurement
• UV-VIS fibre spectrometer USB2000+ (Ocean optics) with range of 200-900 nm for spectrophotometric (transmittance, reflectance) and fluorescent measurement, including suprasil cuvette for UVrange and integration sphere for measurement of diffusion surfaces
• UV-VIS spectrophotometer UV-2600 (Shimadzu) - The compact UV-2600 is a universal, research-grade spectrophotometer that can be used in a wide range of fields, and easily expanded to suit the measurement objective. By using the optional integrating sphere, the measurement wavelength range of the UV-2600 can be extended to the near-infrared region of 1400 nm.
• Thermocamera FLUKE Ti-55 for heat transfer study in material and biological tissues
• Vacuum interaction chambers for laser deposition and hybrid laser deposition (combination of RF discharge, magnetron a laser deposition)
• Magnetron sputtering system Kurt Lesker;
• Ion source Kaufman-Robinson EH200 with maximum energy of 210 eV
• UV irradiation sources (germicide, forensic (Spectroline Optimax OPX-365UV), and for photocatalysis), power meters in UV range: Hamamatsu H9535 with maximum at 250 nm and International Light Technology ILT-1700 with maximum at 365 nm
• Tribometr with corrosion and wear properties measurement extension (Anton Paar Tribometr with rotation and linear testing including liquid environment testing, and potentiostat VersaSTAT3)
• Digital Microscope VHX-7000 (Keyence) - 2D/3D measuring, 4K Ultra-High Accuracy, Change magnification between 20x to 6000x.
• Original Prusa i3 MK3S (Prusa) – possibility to print 3D objects from materials PLA, ABS, PET a Flex PP
• Chemical workplace:
  • Fume chamber, centrifuge, laboratory scale
  • Reverse osmotic device for clean water RiOs-DI 3 UV (Millipore) with resistance > 10 M·cm,
  • Devices for measurement of liquid pH (Inolab 730)
  • Ceramic oven up to 1100 °C
  • Magnetic mixer with heater, mixer with upper stirring
• Oscilloscopes, energy meters for lasers, He-Ne lasers, optical microscope, etc.
• Dry air source
• Optical tables Standa including equipment for needed for construction of optical lines and samples manipulation (Thorlab)

Finances

Projects from grant agencies (GAČR, TAČR) and Student grant competition (SGS CVUT CZ).

Collaborators

Exchange program ERASMUS+ with the University of Kassel, Germany – valid for students and employees.

Firms: BEZNOSKA s.r.o., ProSpon spol. s r.o., Lasak s.r.o., Ippon s.r.o.

Institutes: Institute of Physics of the Czech Academy of Sciences, Institute of Physiology of the Academy of Sciences of the Czech Republic

Scientific outputs of our laboratory:

1. TRAVNICKOVA, M., FILOVA, E., SLEPICKA, P., SLEPICKOVA KASALKOVA, N., KOCOUREK, T., ZALOUDKOVA, M., SUCHY, T., BACAKOVA, L. Titanium-Doped Diamond-like Carbon Layers as a Promising Coating for Joint Replacements Supporting Osteogenic Differentiation of Mesenchymal Stem Cells. International Journal of Molecular Sciences. 2024, 25(5), DOI 10.3390/ijms25052837.
2. ZEMEK, J., HOUDKOVA, J., JIRICEK, P., KOCOUREK, T. Amorphous Carbon Nitride Films: Surface and Subsurface Composition and Bonding. Langmuir. 2024, 40(37), 19538-19547. DOI 10.1021/acs.langmuir.4c02007. 
3. HOLIATKINA, M., SAVCHENKO, D., KOCOUREK, T., PROKHOROV, A., LANČOK, J., KALABUKHOVA, E. Electron Spin Resonance Study of Hydrogen-Free Germanium-Doped Diamond-Like Carbon Films. physica status solidi (b). 2023, 260(1), 1-6. DOI 10.1002/pssb.202200155. 
4. MÁDLOVÁ, A., PÍSAŘÍK, P. The effect of optical filters on the prevention of retinal disease development and progression. In: BENEŠ, P., et al., eds. XII. NATIONAL STUDENT CONFERENCE OF OPTOMETRY AND ORTHOPTICS WITH INTERNATIONAL PARTICIPATION. XIV. NATIONAL STUDENT CONFERENCE OF OPTOMETRY AND ORTHOPTICS WITH INTERNATIONAL PARTICIPATION, Brno, 2023-10-19. Brno: Národní centrum ošetřovatelství a nelékařských zdravotnických oborů, 2023. p. 164-172. 1. ISBN 978-80-7013-621-8.
5. PÍSAŘÍK, P., POLICAR, O., URBÁNKOVÁ, E. Optics and Optometry at CTU, Faculty of Biomedical Engineering. In: PÍSAŘÍK, P., J. REMSA, and M. OTÁHAL, eds. Biomedical Informatics and Engineering 2023. Kladno, 2023-06-15/2023-06-16. Praha: CTU. Faculty of Biomedical Engineering, 2023. p. 2-5. ISBN 978-80-01-07214-1.
6. PÍSAŘÍK, P. BIOCOMPATIBLE CARBON NANOLAYERS FOR COATING LENSES. Lékař a technika. 2022, 52(2), 42-47. ISSN 0301-5491. DOI 10.14311/CTJ.2022.2.02.
7. PÍSAŘÍK, P. Doped diamond-like carbon coatings prepared by hybrid deposition systems for biomedical application. In: PARKMAN, T., R. ŠIROKÁ, and T. JAROŠÍKOVÁ, eds. INSTRUMENTS AND METHODS FOR BIOLOGY AND MEDICINE 2021. Kladno, 2021-06-17. Praha: CTU. Czech Technical University Publishing House, 2021. p. 57-63. ISBN 978-80-01-06917-2.
8. ŠKUBNÍKOVÁ, A., PÍSAŘÍK, P. Tribological properties of contact and spectacle lenses. In: PARKMAN, T., R. ŠIROKÁ, and T. JAROŠÍKOVÁ, eds. INSTRUMENTS AND METHODS FOR BIOLOGY AND MEDICINE 2021. Kladno, 2021-06-17. Praha: CTU. Czech Technical University Publishing House, 2021. p. 73-77. ISBN 978-80-01-06917-2.
9. KOŠINOVÁ, L., PÍSAŘÍK, P., KRČIL, J., KAUFMAN, J., ČÍŽEK, J., BRAJER, J. Study of material properties of titanium alloy Ti6Al4V used for hip arthroplasty after laser treatment. In: PARKMAN, T., R. ŠIROKÁ, and T. JAROŠÍKOVÁ, eds. INSTRUMENTS AND METHODS FOR BIOLOGY AND MEDICINE 2021. Kladno, 2021-06-17. Praha: CTU. Czech Technical University Publishing House, 2021. p. 68-72. ISBN 978-80-01-06917-2.
10. RÉVAYOVÁ, K., PÍSAŘÍK, P. Surface treatment of spectacle lenses. In: BENEŠ, P., et al., eds. XII. NATIONAL STUDENT CONFERENCE OF OPTOMETRY AND ORTHOPTICS WITH INTERNATIONAL PARTICIPATION. Brno, 2021-10-19. Brno: Národní centrum ošetřovatelství a nelékařských zdravotnických oborů, 2021. p. 144-152. 2. ISBN 978-80-7013-611-9.
11. ZEMEK J., JIRICEK, P., HOUDKOVA, J., LEDINSKY, M., JELINEK, M. On the Origin of Reduced Cytotoxicity of Germanium-Doped Diamond-Like Carbon: Role of Top Surface Composition and Bonding. Nanomaterials. 2021, 11(3), 1-10. DOI 10.3390/nano11030567.
12. TYUNINA, M., RUSEVICH, L. L., KOTOMIN, E. A., PACHEROVA, O., KOCOUREK, T., DEJNEKA, A. Epitaxial growth of perovskite oxide films facilitated by oxygen vacancies, J. Mater. Chem. C 2021, 1, 1. DOI: 10.1039/d0tc05750a.
13. ZEMEK, J., HOUDKOVA, J., JIRICEK, P., JELINEK, M., JUREK, K., KOCOUREK, T., LEDINSKY, M. In-depth distribution of elements and chemical bonds in the surface region of calcium-doped diamond-like carbon films.  Appl. Surf. Sci. 2021, 539, 148250(9). DOI: 10.1016/j.apsusc.2020.148250
14. VLCAK, P., FOJT, J., DRAHOKOUPIL, J., BREZINA, V., SEPITKA, J., HORAZDOVSKY, T., MIKSOVSKY, J., CERNY, F., LEBEDA, M., HAUBNER, M. Influence of surface pre-treatment with mechanical polishing, chemical, electrochemical and ion sputter etching on the surface properties, corrosion resistance and MG-63 cell colonization of commercially pure titanium. Materials Science and Engineering C, Biomimetic and Supramolecular Systems. 2020, 115. DOI 10.1016/j.msec.2020.111065.
15. REMSA, J., PÍSAŘÍK, P., DEJNEKA, A., CHRZANOWSKI, A., LÁT, J., KURKIN, O. Coating especially for cooling system surfaces (2020) - Patent no.: 34 486
16. ŠKUBNÍKOVÁ, A., PÍSAŘÍK, P.: Tribological properties of contact lenses. In: Instruments and Methods for Biology and Medicine 2020. Praha: Czech Technical University in Prague, 2020. p. 61-64. ISBN 9788001067963.
17. KOŠINOVÁ, L., PÍSAŘÍK, P., TOLDE, Z., BRAJER, J., KAUFMAN, J.: Improving properties of titanium alloy used for modern hip prosthesis. In: Instruments and Methods for Biology and Medicine 2020. Praha: Czech Technical University in Prague, 2020. p. 40-43. ISBN 9788001067963.
18. TYUNINA, M., PACHEROVA, O., NEPOMNIASHCHAIA, N., VETOKHINA, V., CICHON, S., KOCOUREK, T., DEJNEKA, A. In situ anion-doped epitaxial strontium titanate films. Phys. Chem. Chem. Phys. 2000, 22, 24796(9). DOI: 10.1039/d0cp03644g.
19. TYUNINA, M., PERÄNTIE, J., KOCOUREK, T., SAUKKO, S., JANTUNEN, H., JELINEK, M., DEJNEKA, A. Oxygen vacancy dipoles in strained epitaxial BaTiO3 films. Phys. Rev. Res. 2020, 2, 023056(8). DOI: 10.1103/PhysRevResearch.2.023056.
20. TYUNINA, M., VETOKHINA, O., NEPOMNIASHCHAIA, N., PACHEROVA, O., CICHON, S., KOCOUREK, T., JELINEK, M., DEJNEKA, A. Multiple optical impacts of anion doping in epitaxial barium titanate films. APL Mat. 2020, 8, 071107(6). DOI: 10.1063/5.0007209.
21. JELINEK, M., KOCOUREK, T., JUREK, K., JELINEK, M., SMOLKOVÁ, B., UZHYTCHAK, M., LUNOV, O. Preliminary Study of Ge-DLC Nanocomposite Biomaterials Prepared by Laser Codeposition. Nanomaterials 2019, 9(3), 451(15). DOI: 10.3390/nano9030451.
22. PERÄNTIE, J., SAVINOV, M., KOCOUREK, T., JELÍNEK, M., JANTUNEN, H., DEJNEKA, A., TYUNINA, M. Hybrid polar state in epitaxial (111) PbSc0.5Nb0.5O3 relaxor ferroelectric films. Phys. Rev. Mater. 2019, 3, 014403(7). DOI: PhysRevMaterials.3.014403.
23. PÍSAŘÍK, P., REMSA, J., MIKŠOVSKÝ, J. Improving the properties of spectacle lenses with a diamond-like carbon, Jemná mechanika a optika 11-12 (2019) 338 - 391. 
24. REMSA, J., PÍSAŘÍK, P., DEJNEKA, A., CHRZANOWSKI, A., LÁT, J. Coating especially for cooling system surfaces (2019) - Patent no.: 33 538
25. STUPAKOV, A., PACHEROVA, O., KOCOUREK, T., JELINEK, M., DEJNEKA, A., TYUNINA, M. Negative magnetoresistance in epitaxial films of neodymium nickelate. Phys. Rev. B 2019, 99, 08511(7). DOI: 10.1103/PhysRevB.99.085111.
26. PÍSAŘÍK, P., J. REMSA a J. MIKŠOVSKÝ. Zlepšení vlastností brýlových čoček pomocí diamantu podobného uhlíku. Jemná mechanika a optika. 2019, 2019(11-12), 388-391. ISSN 0447-6441.
27. JELÍNEK, M. et al. Preliminary Study of Ge-DLC Nanocomposite Biomaterials Prepared by Laser Codeposition. Nanomaterials. 2019, 9(3), 1-15. ISSN 2079-4991. DOI: 10.3390/nano9030451.
28. ZEMEK, J. et al. Surface and in-depth distribution of sp(2) and sp(3) coordinated carbon atoms in diamondlike carbon films modified by argon ion beam bombardment during growth. Carbon. 2018, 134 71-79. ISSN 0008-6223. DOI: 10.1016/j.carbon.2018.03.072.
29. JELÍNEK, M. et al. Laser-synthesized nanocrystalline, ferroelectric, bioactive BaTiO3/Pt/FS for bone implants. JOURNAL OF BIOMATERIALS APPLICATIONS. 2018, 32(10), 1464-1475. ISSN 0885-3282. DOI: 10.1177/0885328218768646.
30. KOCOUREK, T. et al. Crystalline Thin Layers of BaTiO3 for Gas Sensors Prepared by PLD. In: Advanced Nanotechnologies for Detection and Defence against CBRN Agents. Springer Nature, 2018. s. 17-30. ISSN 1874-6500. ISBN 978-94-024-1516-2. DOI: 10.1007/978-94-024-1298-7_2.
31. PÍSAŘÍK, P. et al. Antibacterial coatings for biomedical applications. In: Advanced Nanotechnologies for Detection and Defence against CBRN Agents. Springer Nature, 2018. s. 467-476. ISSN 1874-6500. ISBN 978-94-024-1516-2. DOI: 10.1007/978-94-024-1298-7_46.
32. SEKYRKA, O., M. JELÍNEK a J. REMSA. Study of improvement of implants coating with focus on doped biomaterials and laser deposition. In: INSTRUMENTS AND METHODS FOR BIOLOGY AND MEDICINE 2018 Conference Proceedings. Instruments and methods for biology and medicine (IMBM 2018), Kladno, 2018-05-10. Prague: Czech Technical University, 2018. s. 49-52. ISBN 978-80-01-06502-0.
33. KONDRATIEVOVÁ, J., M. JELÍNEK a T. KOCOUREK. Improvement of DLC implant coating using hybrid laser technology and germanium dopation. In: INSTRUMENTS AND METHODS FOR BIOLOGY AND MEDICINE 2018 Conference Proceedings. Instruments and methods for biology and medicine (IMBM 2018), Kladno, 2018-05-10. Prague: Czech Technical University, 2018. s. 33-36. ISBN 978-80-01-06502-0.
34. URZOVÁ, J. a M. JELÍNEK. Determining ablation depth and ablation threshold for tissue using CT imaging. In: INSTRUMENTS AND METHODS FOR BIOLOGY AND MEDICINE 2018 Conference Proceedings. Instruments and methods for biology and medicine (IMBM 2018), Kladno, 2018-05-10. Prague: Czech Technical University, 2018. s. 68-71. ISBN 978-80-01-06502-0.
35. JELÍNEK, M. et al. Technologie výroby hydroxyapatitových kompozitů pomocí pulzní laserové depozice využívající pevnolátkový Nd:YAG laser. [Functional Sample] 2018.
36. PÍSAŘÍK, P., et al. Diamond-like carbon prepared by pulsed laser deposition with ion bombardment: physical properties. Applied Physics A: Materials Science & Processing. 2018, 124(1), s. 1-9. ISSN 1432-0630. DOI: 10.1007/s00339-017-1501-5.
37. ZEMEK, J., et al. Amorphous carbon nanocomposite films doped by titanium: Surface and sub-surface composition and bonding. Diamond and Related Materials. 2018, 81s. 61-69. ISSN 0925-9635. DOI: 10.1016/j.diamond.2017.11.009.
38. URZOVÁ, J. a JELÍNEK, M. Heat transfer modelling of pulsed laser-tissue interaction. Laser Physics. 2018, 28(3), s. 1-5. ISSN 1054-660X. DOI: 10.1088/1555-6611/aa9a9a.
39. JELÍNEK, M., et al. Doped and Multilayer Biocompatible Materials Prepared by Hybrid Laser Deposition. International Journal of Bioscience, Biochemistry and Bioinformatics. 2018, 8(4), s. 252-258. ISSN 2010-3638. DOI: 10.17706/ijbbb.2018.8.4.252-258.
40. PÍSAŘÍK, P., et al. Antibacterial, mechanical and surface properties of Ag-DLC films prepared by dual PLD for medical applications. Materials Science and Engineering C. 2017, 77, pp. 955-962. ISSN 0928-4931. DOI: 10.1016/j.msec.2017.04.005.
41. FILOVA, E., et al. Adhesion and differentiation of Saos-2 osteoblast-like cells on chromium-doped diamond-like carbon coatings. Journal of Materials Science: Materials in Medicine. 2017, 28(17), pp. 1-14. ISSN 0957-4530. DOI: 10.1007/s10856-016-5830-2.
42. JELÍNEK, M., et al. PLD prepared bioactive BaTiO3 films on TiNb implants. Materials Science and Engineering C, Biomimetic and Supramolecular Systems. 2017, 70, pp. 334-339. ISSN 0928-4931. DOI: 10.1016/j.msec.2016.08.072.
43. KOCOUREK, T., et al. Diamond-like carbon layers modified by ion bombardment during growth and researched by Resonant Ultrasound Spectroscopy. Applied Surface Science. 2016, In press. ISSN 0169-4332. DOI: 10.1016/j.apsusc.2017.03.274.
44. JELÍNEK, M., et al. Hybrid laser technology and doped biomaterials. Applied Surface Science. 2016, In press. ISSN 0169-4332. DOI: 10.1016/j.apsusc.2017.03.103.
45. ZEIPL, R., et al. Scanning thermal microscopy of Bi2Te3 and Yb0.19Co4Sb12 thermoelectric films. Applied Physics A: Materials Science and Processing. 2016, 122:155. ISSN 0947-8396. DOI: 10.1007/s00339-016-0017-8.
46. JELÍNEK, M., et al. Thermoelectric nanocrystalline YbCoSb laser prepared layers. Applied Physics A: Materials Science and Processing. 2016, 122:478. ISSN 0947-8396. DOI: 10.1007/s00339-016-9685-7.
47. REMSA, J., et al. Very Smooth FeSb2Te and Ce0.1Fe0.7Co3.3Sb12 Layers Prepared by Modified PLD. Journal of Electronic Materials. 2016, 45(3), pp. 1921-1926. ISSN 0361-5235. DOI: 10.1007/s11664-015-4295-2.
48. JELÍNEK, M., et al. Bonding and bio-properties of hybrid laser/magnetron Cr-enriched. Materials Science and Engineering C, Biomimetic and Supramolecular Systems. 2016, 58(58), pp. 1217-1224. ISSN 0928-4931. DOI: 10.1016/j.msec.2015.09.006.
49. JELÍNEK, M., et al. Dual laser deposition of Ti:DLC composite for implants. Laser Physics. 2016, 26pp. 1-8. ISSN 1054-660X. 10.1088/1054-660X/26/10/105605.
50. JELÍNEK, M., et al. Thermoelectric Simple and Multilayers Prepared by Laser. Journal of Materials Science and Chemical Engineering. 2016, 4(1), pp. 52-64. ISSN 2327-6053.
51. JELÍNEK, M., et al. Hybrid Laser Technology for Creation of Doped Biomedical Layers. Journal of Materials Science and Chemical Engineering. 2016, 4(1), pp. 98-104. ISSN 2327-6053. DOI: 10.4236/msce.2016.41014.
52. ZEIPL, R., et al. Physical Properties of Bi2Te3 Nanolayers. NATO Science for Peace and Security Series A: Chemistry and Biology. 2015, 39pp. 325-331. ISSN 1874-6489. DOI: 10.1007/978-94-017-9697-2_33.
53. JELÍNEK, M., et al. Chromium-doped DLC for implants prepared by laser-magnetron deposition. Materials Science and Engineering C, Biomimetic and Supramolecular Systems. 2015, 46(1.1.2015), pp. 381-386. ISSN 0928-4931. DOI: 10.1016/j.msec.2014.10.035.
54. PÍSAŘÍK, P., et al. Influence of diamond and graphite bonds on mechanical properties of DLC thin films. In: Journal of Physics Conference Series. 7th International Workshop on Decoherence, Information, Complexity and Entropy (DICE) - Spacetime - Matter - Quantum Mechanics. Castiglioncello, 15.09.2014 - 19.09.2014. Bristol: IOP Publishing Ltd. 2015, pp. 1-6. ISSN 1742-6588. DOI: 10.1088/1742-6596/594/1/012008.
55. PÍSAŘÍK, P., et al. Chromium doped diamond like carbon films deposited by dual pulsed laser deposition. Applied Physics A: Materials Science and Processing. 2014, 117(1), pp. 83-88. ISSN 0947-8396. DOI: 10.1007/s00339-013-8206-1.
56. JELÍNEK, M., et al. Preliminary comparative study of laser-prepared DLC and Cr-doped DLC for bacteria adhesion. Applied Physics A: Materials Science and Processing. 2014, 116(3), pp. 1437-1443. ISSN 0947-8396. DOI: 10.1007/s00339-014-8256-z.
57. SOCOL, M., et al. Organic heterostructures based on arylenevinylene oligomers deposited by MAPLE. Applied Surface Science. 2014, 302(0), pp. 216-222. ISSN 0169-4332. DOI: 10.1016/j.apsusc.2013.12.091.
58. MIKŠOVSKÝ, J., et al. Cell adhesion and growth on ultrananocrystalline diamond and diamond-like carbon films after different surface modifications. Applied Surface Science. 2014, 297pp. 95-102. ISSN 0169-4332. DOI: 10.1016/j.apsusc.2014.01.085.
59. ZEZULOVÁ, M., et al. Polycrystalline LiNbO3 thin films characterized by infrared and Raman spectroscopy. Laser Physics. 2014, 24(2), pp. 1-4. ISSN 1054-660X. DOI: 10.1088/1054-660X/24/2/025701.
60. KOCOUREK, T., et al. Silver doped metal layers for medical applications. Laser Physics. 2014, 24(8), pp. 1-7. ISSN 1054-660X. 10.1088/1054-660X/24/8/085602.
61. KOCOUREK, T., et al. Silver doped metal layers for medical applications. In: Journal of Physics: Conference Series, vol. 497. 22nd International Laser Physics. Prague, 15.07.2013 - 19.07.2013. Bristol: IOP Publishing Ltd. 2014, pp. 1-9. ISSN 1742-6588. DOI: 10.1088/1742-6596/497/1/012021.
62. KYMPLOVÁ, J., et al. Assessment of the Suitability of Excimer Lasers in Treating Onychomycosis. In: Journal of Physics: Conference Series, vol. 497. 22nd International Laser Physics. Prague, 15.07.2013 - 19.07.2013. Bristol: IOP Publishing Ltd. 2014, pp. 1-13. ISSN 1742-6588. DOI: 10.1088/1742-6596/497/1/012022.
63. ZEIPL, R., et al. Properties of thermoelectric Ce0.09Fe0.67Co 3.33Sb12/FeSb2Te multi-layered structures prepared by laser ablation. In: Journal of Physics: Conference Series, vol. 497. 22nd International Laser Physics. Prague, 15.07.2013 - 19.07.2013. Bristol: IOP Publishing Ltd. 2014, pp. 1-10. ISSN 1742-6588. 10.1088/1742-6596/497/1/012038.
64. JELÍNEK, M., et al. Comparison of the surface properties of DLC and ultrananocrystalline diamond films with respect to their bio-applications. PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE. 2013, 210(10), pp. 2106-2110. ISSN 1862-6300. DOI: 10.1002/pssa.201228713.
65. JELÍNEK, M., et al. Composition, XRD and morphology study of laser prepared LiNbO3 films. Applied Physics A: Materials Science and Processing. 2013, 110(4), pp. 883-888. ISSN 0947-8396. DOI: 10.1007/s00339-012-7191-0.
66. JELÍNEK, M., et al. Influence of ion bombardment on growth and properties of PLD created DLC films. Applied Physics A: Materials Science and Processing. 2013, 110(4), pp. 943-947. ISSN 0947-8396. DOI: 10.1007/s00339-012-7215-9.
67. PÍSAŘÍK, P., et al. Study of optical properties and biocompatibility of DLC films characterized by sp3 bonds. Applied Physics A: Materials Science and Processing. 2013, 112(1), pp. 143-148. ISSN 0947-8396. DOI: 10.1007/s00339-012-7216-8.
68. JELÍNEK, M., et al. Antibacterial, cytotoxicity and physical properties of laser — Silver doped hydroxyapatite layers. Materials Science and Engineering C, Biomimetic and Supramolecular Systems. 2013, 33(3), pp. 1242-1246. ISSN 0928-4931. DOI: 10.1016/j.msec.2012.12.018.
69. ZEIPL, R., et al. Properties of thermoelectric Ce0.09Fe0.67Co3.33Sb12/FeSb2Te multi-layered structures prepared by laser ablation. Thin Solid Films. 2013, 548(0), pp. 590-596. ISSN 0040-6090. DOI: 10.1016/j.tsf.2013.09.068.
70. JELÍNEK, M., et al. Optical properties of laser-prepared Er- and Er,Yb-doped LiNbO3 waveguiding layers. Laser Physics. 2013, 0(23), pp. 1-5. ISSN 1054-660X. DOI: 10.1088/1054-660X/23/10/105819.
71. JELÍNEK, M. Hybrid laser technology for biomaterials. In: JELÍNKOVÁ, H., ed. Lasers for medical applications. Abington Camprige: Woodhead Publishing. 2013, pp. 704-724. 1. vol. 37. ISBN 9780857092373. DOI: 10.1533/9780857097545.4.704.
72. JELÍNEK, M., PODLAHA, J., and KOCOUREK, T. DLC Coated Textile Vascular Prostheses Tested in Sheep. In: Advanced Materials Research. International Conference on Biomaterial and Bioengineering. Hong Kong, 19.12.2012 - 20.12.2012. Durnten-Zurich: Trans Tech Publications. 2013, pp. 20-24. ISSN 1022-6680. ISBN 9783037855973. DOI: 10.4028/www.scientific.net/AMR.647.20.
73. MIKŠOVSKÝ, J., et al. Micro and Macro Scratch and Microhardness Study of Biocompatible DLC and TiO2 Films Prepared by Laser. In: Advanced Materials Research. International Conference on Biomaterial and Bioengineering. Hong Kong, 19.12.2012 - 20.12.2012. Durnten-Zurich: Trans Tech Publications. 2013, pp. 25-29. ISSN 1022-6680. ISBN 9783037855973. DOI: 10.4028/www.scientific.net/AMR.647.25.
74. URZOVÁ, J., et al. Treatment of Onychomycosis Using Radiation of Excimer Laser. In: Advanced Materials Research. International Conference on Biomaterial and Bioengineering. Hong Kong, 19.12.2012 - 20.12.2012. Durnten-Zurich: Trans Tech Publications. 2013, pp. 636-641. ISSN 1022-6680. ISBN 9783037855973.
75. ZEIPL, R., et al. Thermoelectric Properties of Ce0.09Fe0.67Co3.33Sb12/FeSb2Te Multi-Layered Structures. Journal of Computer and Communications. 2013, 1(7), pp. 1-4. ISSN 2327-5219.
76. KOCOUREK, T., et al. Silver-Doped Layers of Implants Prepared by Pulsed Laser Deposition. Journal of Computer and Communications. 2013, 1(7), pp. 59-61. ISSN 2327-5219.JELÍNEK, M. Functional planar thin film optical waveguide lasers. Laser Physics Letters. 2012, 9(2), pp. 91-99. ISSN 1612-2011.
77. REMSA, J., JELÍNEK, M., and MIKŠOVSKÝ, J. PLD and RF discharge combination used for preparation of photocatalytic TiO2 layers. Applied Surface Science. 2012, 258(23), pp. 9333-9336. ISSN 0169-4332. DOI: 10.1016/j.apsusc.2012.02.042.
78. KUTÍLEK, P., et al. The evaluation and comparison of the practical adhesion strength of biocompatible nano and micro thin films by fuzzy logic [online]. In: 35th International Conference Telecommunications and Signal Processing. 35th International Conference on Telecommunications and Signal Processing. Prague, 03.07.2012 - 04.07.2012. Brno: VUT v Brně, Fakulta elektrotechniky a komunikačních technologií. 2012, pp. 489-493. ISBN 978-1-4673-1118-2.
79. PROSECKÁ, E., et al. Thin-Layer Hydroxyapatite Deposition on a Nanofiber Surface StimulatesMesenchymal Stem Cell Proliferation and Their Differentiation into Osteoblasts. Journal of Biomedicine and Biotechnology. 2012, 2012(0), pp. 1-10. ISSN 1110-7243. DOI: 10.1155/2012/428503.
80. JELÍNEK, M., et al. MAPLE activities and applications in gas sensors. Applied Physics A: Materials Science and Processing. 2011, 105(3), pp. 643-649. ISSN 0947-8396. DOI: 10.1007/s00339-011-6629-0.
81. KOCOUREK, T., et al. Evaluation of elastic properties of DLC layers using resonant ultrasound spectroscopy and AFM nanoindentation. Surface & Coatings Technology. 2011, 205(2), pp. 67-70. ISSN 0257-8972. DOI: 10.1016/j.surfcoat.2011.01.038.
82. JELÍNEK, M., REMSA, J., and ZEZULOVÁ, M. Laser Deposition of TiO2 for Urethral Catether. In: Proc. of SPIE Vol. 7747 - 16th International School on Quantum Electronics: Laser Physics and Applications. 16th International School on Quantum Electronics: Laser Physics and Applications. Nessebar, 20.09.2011 - 24.09.2011. Bellingham: SPIE. 2011, pp. 774703-1-774703-8. ISSN 0277-786X. ISBN 978-0-8194-8237-2. DOI: 10.1117/12.885085.
83. ZEZULOVÁ, M., et al. Study of Thin Films of LiNbO3 Using FTIR and Raman Spektroscopy. In: Proceedings of SPIE Vol. 8306 - Photonics, Devices, and Systems V. Photonics Prague 2011. Praha, 24.08.2011 - 26.08.2011. Bellingham: SPIE. 2011, pp. 91. ISSN 0277-786X. ISBN 978-0-8194-8953-1. 10.1117/12.910590.
84. JELÍNEK, M., et al. Biomedical Properties of Laser Prepared Silver-Doped Hydroxyapatite. Laser Physics. 2011, 21(7), pp. 1265-1269. ISSN 1054-660X. 10.1134/S1054660X11130159.
85. JELÍNEK, M., et al. Antibacterial Properties of Ag-Doped Hydroxyapatite Layers Prepared by PLD Method. Applied Physics A: Materials Science and Processing. 2010, 101(4), pp. 615-620. ISSN 0947-8396. DOI: 10.1007/s00339-010-5911-x
86. JELÍNEK, M., et al. Diamond/Graphite Content and Biocompatibility of DLC Films Fabricated by PLD. Applied Physics A: Materials Science and Processing. 2010, 101(4), pp. 579-583. ISSN 0947-8396. DOI: 10.1007/s00339-010-5912-9.
87. JELÍNEK, M., et al. Conductive Gas Sensors Prepared Using PLD. In: Proceedings of the NATO Advanced Study Institute on Nanotechnological Basic for Advanced Sensors. Science for Peace and Security. Sozopol, 30.05.2010 - 11.06.2010. Dordrecht: Springer. 2010, pp. 391-399. ISBN 978-94-007-0903-4. 10.1007/978-94-007-0903-4_40.
88. JELÍNEK, M., et al. Biocompatibility and Sp3/Sp2 Ratio of Laser Created DLC Films. Materials Science and Engineering: B. 2010, 163(1-3), pp. 89-93. ISSN 0921-5107. DOI: 10.1016/j.mseb.2010.01.010.
89. ZEIPL, R., et al. Properties of Thin N-type Yb0.14Co4Sb12 and P-type Ce0.09Fe0.67Co3.33Sb12 Skutterudite Layers Prepared by Laser Ablation. Journal of Vacuum Science and Technology A. 2010, 28(4), pp. 523-527. ISSN 0734-2101. DOI: 10.1116/1.34258037.
90. JELÍNEK, M., et al. Doped Biocompatible Layers Prepared by Laser. Laser Physics. 2010, 20(3), pp. 562-567. ISSN 1054-660X. 10.1134/S1054660X10050087.
91. JELÍNEK, M., et al. SiCx Layers Prepared by Hybrid Laser Deposition and PLD. Plasma Processes and Polymers. 2009, 6(1), 366-369. ISSN 1612-8850. 10.1002/ppap.200930803.
92. KOPECKÝ, D., et al. Polypyrrole Thin Films for Gas Sensors Prepared by Matrix-Assisted Pulsed Laser Evaporation Technology: Effect of Deposition Parameters on Material Properties. Thin Solid Films. 2009, 517(6), 2083-2087. ISSN 0040-6090. DOI: 10.1016/j.tsf.2008.10.047.
93. JELÍNEK, M., et al. Hybrid laser-magnetron technology for carbon composite coating. Laser Physics. 2009, 19(2), 149-153. ISSN 1054-660X. 10.1134/S1054660X09020017.
94. JELÍNEK, M., et al. Highly oriented crystalline Er:YAG and Er:YAP layers prepared by PLD and annealing. Applied Surface Science. 2009, 255(10), 5292-5294. ISSN 0169-4332. DOI: 10.1016/j.apsusc.2008.08.037.
95. JELÍNEK, M., et al. Pulsed Laser Deposition: Passive and Active Waveguides. International Journal of Materials & Product Technology. 2009, 34(4), 438-453. ISSN 0268-1900.
96. JELÍNEK, M. Growth of Optical Waveguides by Pulsed Laser Deposition. Laser Physics. 2009, 19(2), 265-273. ISSN 1054-660X. 10.1134/S1054660X09020194.
97. JELÍNEK, M., et al. Thin SiCx Layers Prepared by Hybrid Laser-Magnetron Deposition, Applied Physics A: Materials Science and Processing. 2008, 93(3), 633-637. ISSN 0947-8396. DOI: 10.1007/s00339-008-4727-4.
98. KOCOUREK, T., et al. DLC Coating of Textile Blood Vessels using PLD. Applied Physics A: Materials Science and Processing. 2008, 93(3), 627-632. ISSN 0947-8396. DOI: 10.1007/s00339-008-4728-3.
99. JELÍNEK, M., et al. Nanotechnologie Praha: Technologické centrum AV ČR, 2008.

Organisation of conferences and workshops

• Biomedical Informatics and Engineering 2023 (Kladno) - The conference was divided into three thematic sessions. The first focused on biomedical informatics and engineering, presenting new technologies and approaches in data processing and medical devices. The second session covered biomaterials in medicine and emerging trends in implantology, with an emphasis on innovations in implants and their interactions with the human body. The third part was dedicated to optics and optometry, highlighting the latest research and applications in eye care.
• Progressive materials and optics 2020 (Kladno) - The conference focused on the latest findings in the field of optics and optometry - refraction, contact lenses and ophthalmology. It also included a session dealing with the development of new materials and measurement possibilities in optics and optometry, modern eye examination procedures and other optical phenomena related to this issue. The proceedings of Progressive Materials and Optics 2020 were published.
• Optics and Optometry Forum 2017 (Kladno)
• Human Biomechanics 2016 (Kladno)
• Progressive Biomedical Materials and Technologies, 2015 (Kladno)
• LPHYS'13 The 22nd International Laser Physics Workshop, 2013 (Prague)

Who are we?

We are a multidisciplinary team dedicated to research and development in the field of medical technologies in the context of space applications. We are primarily concerned with specific technologies for analog missions and space in relation to the human factor. The above includes the design and development of software and mechatronic technologies to meet certain quality and robustness requirements in environments such as ICE (isolated, confined, and extreme) environments. Noteworthy is the development of systems and methods following ECSS (European Cooperation for Space Standardization) technical standards. We take advantage of the fact that the FBMI CTU brings together specialists with expertise in computer science, robotics, rocketry, and medicine with experience in simulation, measurement, processing, and analysis of medical and mechatronic data.

What research we do:

We are involved in the development of methods and systems for recording and evaluation of biomedical, simulator and environmental data, software implementation and design of mechatronic parts of assistive and ergonomic aids for analog and space applications.

Team members:

Ing. et Ing. Jan Hejda, Ph.D.
doc. Ing. Patrik Kutílek, M.Sc., Ph.D.; Ing. Lýdie Leová,; Ing. Petr Volf; Ing. Marek Sokol, Bc. Jan Tonner, Ing. Veronika Kotolová, Bc. Blanka Sliacká, etc.

What is it for?

Software for recording and evaluating physiological, environmental and ergonomic data and mechatronic systems form an essential part of many means for training, transport and human stay in space. In particular, such systems have significant applications in diagnosing and managing physical fitness in ICE (isolated, confined, and extreme) environments. Methods of processing and evaluation of physiological, environmental, and simulator data are used to diagnose the state of the musculoskeletal system, nervous system, and other systems of the human body. The physical and mental state of crews during analog training and space missions affects mission safety and the quality of mission performance. 
Systems for such applications allow long-term and short-term monitoring of crews' health status and identify possible health status problems and prevent dangerous situations during mission tasks. These systems also form an essential part of the assistance aids. Development and research are being carried out in accordance with the ECSS. Promising assistive devices include 'smart' surveillance sensor systems for recording biomedical data, MoCap systems or exoskeletons to support training and demanding tasks, etc. To guarantee the safe function of the devices for demanding applications, it is also necessary to evaluate their impact on the body, which is related to the appropriate selection and testing of the designs in challenging environments with low or high pressure, humidity, temperature and overload.

Selected projects:

• TACR DELTA TM04000062 (2023-2025) Development of the platform for maintaining and monitoring the physical conditions in isolated, confined and extreme environments
• TACR ETA TL05000228 (2021-2023) Tool for assessment of personal characteristics and external factors to improve efficiency and collaboration of the team during a long-time stay in “Integrated Collaboration Environment (ICE)”
• TACR TREND FW01010444 (2020-2022) Reserch and development of synthetic device with artificial intelligence focused on pilot preselection and screening
• TACR ZETA TJ02000036 (2019-2021) Back behind the Wheel - Diagnostic and rehabilitation tool for people after brain injury

Selected publications:

1. Sokol M., Hejda J., Volf P., Kutílek P. Multimodal Emotion Recognition Through a Hybrid CNN-GCN Model Integrating Neuroimaging and Physiological Data (2025) IFMBE Proceedings, 118 IFMBE, pp. 399 - 405
2. Adolf J., Segal Y., Turna M., Nováková T., Doležal J., Kutílek P., Hejda J., Hadar O., Lhotská L. Evaluation of functional tests performance using a camera-based and machine learning approach (2023) PLoS ONE, 18 (11 NOVEMBER), art. no. e0288279
3. Sokol M., Volf P., Hejda J., Leová L., Hýbl J., Schmirler M., Suchý J., Procházka R., Charvát M., Seitlová K., Dolejš M., Schneider J., Kutílek P. DIANA: An underwater analog space mission (2025) Acta Astronautica, 226, pp. 349 - 360
4. Kutilek P., Karavaev A., Hejda J., Krivanek V., Hyb J., Hájková S., Volf P. Control System and User Interface of Cooling Module for Braces
   (2021) IFMBE Proceedings, 80, pp. 286 - 293
5. Hejda J., Kutilek P., Volf P., Sokol M., Leova L., Tonner J., Hejskova M., Kotolova V., Rozloznik M., Sugiarto T., Lin Y.-J., Huang K.-L., Hsu W.-C. Wearable System for Monitoring the Physical Conditions in Isolated, Confined and Extreme Environments (2024) Proceedings of the 2024 21st International Conference on Mechatronics - Mechatronika, ME 2024,
6. Hejda J., Volf P., Sokol M., Leová L., Kutílek P. Spinal Curvature Estimation Using Low-Cost Portable Sensors
   (2025) IFMBE Proceedings, 118 IFMBE, pp. 32 - 37
7. Volf P., Hsu W.-C., Hejda J., Lin Y.-J., Kutilek P., Sugiarto T., Sokol M., Leova L., Tsai H.-L., Chang S.-Y., Tang L.-X. Use of Nonlinear Analysis Methods for Visual Evaluation and Graphical Representation of Bilateral Jump Landing Tasks (2023) 2023 IEEE 5th Eurasia Conference on Biomedical Engineering, Healthcare and Sustainability, ECBIOS 2023, pp. 75 - 78, Cited 0 times.
8. Rozloznik M., Schneider J., Hejda J., Kutilek P., Volf P., Sokol M., Hsu W.C., Huang K.L., Lin Y.J., Sugiarto T., Tang L.X., Lin Y.C. Tool for real-time monitoring and analysis of the exercise in the ICE environment (2024) Proceedings of the International Astronautical Congress, IAC, 2, pp. 798 - 801, Cited 0 times.
9. Volf P., Hejda J., Sokol M., Leová L., Lin Y.-J., Sugiarto T., Kutílek P. RGB-D Motion Capture System for Monitoring Health Conditions of Employees in Physically Demanding Professions (2025) IFMBE Proceedings, 118 IFMBE, pp. 38 - 44
10. Žarković, D., Šorfová, M., Tufano, J.J., Kutílek, P., Vítečková, S., Ravnik, D., Groleger-Sršen, K., Cikajlo, I., Otáhal, J.; Gait changes following robot-assisted gait training in children with cerebral palsy (2021) Physiological research, 70 (S3), pp. S397-S408.
11. Leova, L., Cubanova, S., Kutilek, P., Volf, P., Hejda, J., Hybl, J., Stastny, P., Vagner, M., Krivanek, V. Current State and Design Recommendations of Exoskeletons of Lower Limbs in Military Applications (2022) Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics), 13207 LNCS, pp. 452-463. 
12. Mitriková, A., Hejda, J., Volf, P., Bačíková, M., Oberman, Č., Rusnáková, K., Braunová, M., Kutílek, P. Pilot Study of Application of a Hybrid Transportable System for Postural Stability Measurement in Military Professions (2021) IFMBE Proceedings, 80, pp. 968-975. 
13. Kutilek, P., Hejda, J., Volf, P., Krivanek, V., Cicmanec, L., Hana, K., Smrcka, P., Fajnerova, I.Evaluation of Psychological Load of Air Defense Members by Physiological Data Monitoring Compared to the Questionnaire Evaluation Method (2020) Proceedings of the 2020 19th International Conference on Mechatronics - Mechatronika, ME 2020, art. no. 9286626,
14. Volf, P., Bernaskova, K., Hejda, J., Francova, A., Kutilek, P., Hybl, J., Hourova, M. Car Simulator for Selection and Screening of Patients after Brain Injury (2020) Proceedings of the 2020 19th International Conference on Mechatronics - Mechatronika, ME 2020, art. no. 9286600,
15. Volf, P., Kutilek, P., Hejda, J., Viteckova, S., Smrcka, P., Hana, K., Svoboda, Z., Krivanek, V. Methods for kinematic analysis of human movement in military applications: A review of current and prospective methods (2020) Lekar a Technika, 49 (4), pp. 125-135. 
16. Hejda, J., Volf, P., Bačíková, M., Bar, N., Oberman, C., Rusnáková, K., Braunová, M., Kutílek, P. Design of a hybrid portable system for measuring the position of the spine, pelvis and center of gravity of the body (2020) IFMBE Proceedings, 76, pp. 622-633.
17. Hejda, J., Volf, P., Mejstřík, J., Hýbl, J., Tvrzník, A., Gerych, D., Michálek, T., Oberman, Č., Bolek, E., Kutílek, P. Design of device for measuring the load of cross-country ski poles (2020) IFMBE Proceedings, 76, pp. 640-649. 
18. Leova, L., Cubanova, S., Kutilek, P., Volf, P., Hejda, J., Hybl, J., Stastny, P., Vagner, M., Krivanek, V. Current State and Design Recommendations of Exoskeletons of Lower Limbs in Military Applications (2022) Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics), 13207 LNCS, pp. 452-463. 
19. Mitriková, A., Hejda, J., Volf, P., Bačíková, M., Oberman, Č., Rusnáková, K., Braunová, M., Kutílek, P. Pilot Study of Application of a Hybrid Transportable System for Postural Stability Measurement in Military Professions (2021) IFMBE Proceedings, 80, pp. 968-975. 
20. Kutilek, P., Hejda, J., Volf, P., Krivanek, V., Cicmanec, L., Hana, K., Smrcka, P., Fajnerova, I.Evaluation of Psychological Load of Air Defense Members by Physiological Data Monitoring Compared to the Questionnaire Evaluation Method (2020) Proceedings of the 2020 19th International Conference on Mechatronics - Mechatronika, ME 2020, art. no. 9286626,
21. Volf, P., Bernaskova, K., Hejda, J., Francova, A., Kutilek, P., Hybl, J., Hourova, M. Car Simulator for Selection and Screening of Patients after Brain Injury (2020) Proceedings of the 2020 19th International Conference on Mechatronics - Mechatronika, ME 2020, art. no. 9286600,
22. Volf, P., Kutilek, P., Hejda, J., Viteckova, S., Smrcka, P., Hana, K., Svoboda, Z., Krivanek, V. Methods for kinematic analysis of human movement in military applications: A review of current and prospective methods (2020) Lekar a Technika, 49 (4), pp. 125-135. 
23. Hejda, J., Volf, P., Bačíková, M., Bar, N., Oberman, C., Rusnáková, K., Braunová, M., Kutílek, P. Design of a hybrid portable system for measuring the position of the spine, pelvis and center of gravity of the body (2020) IFMBE Proceedings, 76, pp. 622-633.
24. Hejda, J., Volf, P., Mejstřík, J., Hýbl, J., Tvrzník, A., Gerych, D., Michálek, T., Oberman, Č., Bolek, E., Kutílek, P. Design of device for measuring the load of cross-country ski poles (2020) IFMBE Proceedings, 76, pp. 640-649. 
25. Kutilek, P., Volf, P., Sedova, K., Hejda, J., Krivanek, V., Stehlík, M., Rusnakova, K., Kozlova, S., Braunova, M. Heart rate variability during fighter pilot training (2019) ICMT 2019 - 7th International Conference on Military Technologies, Proceedings, art. no. 8870071, . Cited 1 time.
26. Volf, P., Stehlik, M., Kutilek, P., Kloudova, G., Rusnakova, K., Kozlova, S., Braunova, M., Hejda, J., Krivanek, V., Doskocil, R. Brain electrical activity mapping in military pilots during simulator trainings (2019) ICMT 2019 - 7th International Conference on Military Technologies, Proceedings, art. no. 8870112, . 
27. Kutilek, P., Volf, P., Hejda, J., Smrcka, P., Adolf, J., Krivanek, V., Lhotska, L., Hana, K., Doskocil, R., Kacer, J., Cicmanec, L. Non-contact measurement systems for physiological data monitoring of military pilots during training on simulators: Review and application (2019) ICMT 2019 - 7th International Conference on Military Technologies, Proceedings, art. no. 8870115, 
28. Kacer, J., Krivanek, V., Cicmanec, L., Kutilek, P., Farlik, J., Hejda, J., Viteckova, S., Volf, P., Hana, K., Smrcka, P. Physiological data monitoring of members of air forces during training on simulators (2019) IFMBE Proceedings, 68 (3), pp. 855-860. Cited 5 times.
29. Krivanek, V., Kutilek, P., Doskocil, R., Farlik, J., Casar, J., Hejda, J., Viteckova, S., Volf, P., Smrcka, P.Evaluation methodology and measurement of physiological data to determine operational preparedness of air defense staff: Preliminary results (2019) IFMBE Proceedings, 68 (1), pp. 351-355. Cited 1 time.
30. D'Angeles, A., Kutilek, P., Krivanek, V., Farlik, J., Hejda, J., Volf, P., Smrcka, P., Doskocil, R., Casar, J. Non-linear analysis of trunk movement of air defense staff: Pilot study (2018) Gait and Posture, 65, pp. 505-506. Cited 1 time.
31. Volf, P., Kutilek, P., Hejda, J., Smrcka, P., Krivanek, V., Doskocil, R., Farlik, J., Casar, J., Klikova, M. Heart Rate Variability during Long-Term Air Traffic Control in Cadets (2018) 2018 41st International Conference on Telecommunications and Signal Processing, TSP 2018, art. no. 8441257,
32. Tuan Bui, M., Doskocil, R., Krivanek, V., Hien Ha, T., Bergeon, Y., Kutilek, P. Indirect method usage of distance and error measurement by single optical cameras (2018) Advances in Military Technology, 13 (2), pp. 209-221. 
33. Kacer, J., Kutilek, P., Krivanek, V., Doskocil, R., Smrcka, P., Krupka, Z. Measurement and modelling of the behavior of military pilots (2018) Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics), 10756 LNCS, pp. 434-449.
34. Kutilek, P., Volf, P., Viteckova, S., Smrcka, P., Krivanek, V., Lhotska, L., Hana, K., Doskocil, R., Navratil, L., Hon, Z., Stefek, A. Wearable systems for monitoring the health condition of soldiers: Review and application (2017) ICMT 2017 - 6th International Conference on Military Technologies, art. no. 7988856, pp. 748-752. 
35. Bui, M.T., Doskocil, R., Krivanek, V., Ha, T.H., Bergeon, Y.T., Kutilek, P. Indirect method to estimate distance measurement based on single visual cameras (2017) ICMT 2017 - 6th International Conference on Military Technologies, art. no. 7988846, pp. 695-700. 
36. Kliment, R., Smrčka, P., Hána, K., Schlenker, J., Socha, V., Socha, L., Kutílek, P. Wearable modular telemetry system for the integrated rescue system operational use (2017) Journal of Sensors, 2017, art. no. 9034253, 
37. Kutilek, P., Mares, J., Hybl, J., Socha, V., Schlenker, J., Stefek, A. Myoelectric arm using artificial neural networks to reduce cognitive load of the user (2017) Neural Computing and Applications, 28 (2), pp. 419-427. 
38. Stary, V., Doskocil, R., Krivanek, V., Kutilek, P., Stefek, A. Missile guidance systems for UAS landing application (2017) Proceedings of the 2016 17th International Conference on Mechatronics - Mechatronika, ME 2016, art. no. 7827863,
39. Kutílek, P., Volf, P., Viteckova, S., Smrcka, P., Lhotska, L., Hana, K., Krivanek, V., Doskocil, R., Navratil, L., Hon, Z., Stefek, A.Wearable systems and methods for monitoring psychological and physical condition of soldiers (2017) Advances in Military Technology, 12 (2), pp. 259-280.
40. Volf, P., Kutílek, P., Hozman, J., Cerný, R., Koukolík, T., Hejda, J.System for measuring kinematics of vestibular system movements in neurological practice (2016) Acta Polytechnica, 56 (4), pp. 336-343.

Mgr. Ksenia Sedova, Ph.D.
___________________________________
Associate Professor
Department of Biomedical Technology
Faculty of Biomedical Engineering
Czech Technical University in Prague
email: ksenia.sedova@fbmi.cvut.cz
 

Associate Professor at the Department of Biomedical Technology. In terms of expertise, she has been researching electrophysiological processes in the heart for more than 15 years. Currently, she leads an international team that conducts research in cardiovascular pathophysiology and related clinical studies, basic research and simulation technologies. She was the local organizer of the 18th International STAFF-MALT Symposium 2022 (Sept 14-17, 2022, Prague, Czech Republic) and has been a member of the STAFF-MALT meetings organization committee since 2022.

Team:

Ksenia Sedova, PhD – team leader.
Jan Matějka, PhD – team member. He deals with the design and implementation of an automatic algorithm for processing electrical signals of the heart.
Jan Azarov, PhD – team member. He deals with the analysis of electrophysiological parameters of the heart in terms of their diagnostic significance.
Anastasia Sedova, PhD - team member. She is involved in the processing of cardiac electrical potentials, contributing to the validation of an automatic algorithm for signal analysis.
Tamerlan Yusifov, Bc., MSc. – student of the doctoral study programme Biomedical Engineering at the Faculty of Biomedical Engineering of the Czech Technical University in Prague. She deals with the pathophysiological significance of electrocardiographic parameters of electrical dyssynchrony.
Peter van Dam, PhD (University Medical Center Utrecht, Utrecht, The Netherlands; ECG Excellence BV, Nieuwerbrug, The Netherlands) – team member. It provides software for electrocardiographic data processing and deals with inverse electrophysiological problem solving and computer simulations of electrical processes in the heart. 
Agnese Sbrollini, PhD (Università Politecnica della Marche, Ancona, Italy) – team member. She deals with the processing of ECG signals.
Prof. Josef Kautzner, MD, PhD, FESC (Department of Cardiology, Institute for Clinical and Experimental Medicine, Prague, Czech Republic) – expert consultant of the team.

Research projects:

1. Application of BSPM and 12-lead ECG for assessment of electrical dyssynchrony in cardiac resynchronization therapy

Multidisciplinary research project is based on collaboration with the Department of Cardiology, Institute for Clinical and Experimental Medicine (prof. Josef Kautzner, MD, PhD, FESC). Computer simulations using patient-specific heart-torso model and inverse solution approach for reconstruction of myocardial potentials are applied in cooperation with the Department of Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands (Peter van Dam, Ph.D.). Processing of ECG signals and development of new interactive application Point2ECG for automatic detection of the referent points in ECG signals for the assessment of ECG parameters of ventricular depolarization and repolarization is realized with the colleagues from Università Politecnica della Marche, Ancona, Italy (Agnese Sbrollini, PhD, prof. Laura Burattini, PhD).

Publications:

1. Sedova K, Repin K, Donin G, Van Dam P, Kautzner J. Clinical utility of body surface potential mapping in CRT patients. Arrhythm Electrophysiol Rev 2021; 10(2):113-119.
2. Sedova KA, van Dam PM, Sbrollini A, Burattini L, Necasova L, Blahova M, Bocek J, Sramko M, Kautzner J. Assessment of electrical dyssynchrony in cardiac resynchronization therapy: 12-lead electrocardiogram vs. 96-lead body surface map.Europace 2023; 25(2):554-560.
3. Sedova K, Van Dam PM, Blahova M, Necasova L, Kautzner J. Localization of the ventricular pacing site from BSPM and standard 12-lead ECG: a comparison study. Scientific Reports 2023; 13(1):1-11.
4. Sramko M, Kryze L, Kukla J, Necasova L, Wunschova H, Bocek J, Sedova K, Kautzner J. Acute hemodynamic effect of a novel dual-vein, multisite biventricular pacing configuration. JACC Clin Electrophysiol 2023; 9(11):2329-2338.
5. Sedova K, Azarov JE, Van Dam PM, Necasova L, Kukla J, Sramko M, Kryze L, Kautzner J. CineECG Repolarization Gradients Predict Acute Hemodynamic Response in CRT Patients. J Cardiovasc Electrophysiol 2025;36(2):338-346.

2. Prediction of ventricular fibrillation in STEMI patients

This clinical research is devoted to the solution of two main problems: 1. search for the electrocardiographic parameters of depolarization and repolarization that are associated with the development of ventricular fibrillation in patients with acute coronary syndrome, 2. development of an algorithm for the automated determination of these parameters.

Publications:

1. Sedova KA, Demidova MM, Azarov JE, Hejda J, Carlson J, Bernikova OG, Arteyeva N, Erlinge D, Platonov PG. Terminal T-wave inversion predicts reperfusion tachyarrhythmias in STEMI. J Electrocardiol 2022; 71:28-31.
2. Sedova K, Galinyte V, Arteyeva N, Hejda J, Bernikova O, Kneppo P, Azarov J. Multi-lead vs single-lead Tpeak-Tend interval measurements for prediction of reperfusion ventricular tachyarrhythmias. J Cardiovasc Electrophysiol 2019; 30(10):2090-2097.

3. Mechanisms of ventricular fibrillation and modification of the arrhythmogenic substrate

The main objective of this basic research is to determine the role of different electrophysiological parameters in the development of ventricular fibrillation and then develop a new tool for the stratification the risks of ventricular fibrillation based on the analysis of myocardial electrophysiological signals.

Publications:

1. Bernikova OG, Durkina AV, Sedova KA, Azarov JE. Determinants of reperfusion arrhythmias: action potential duration versus dispersion of repolarization. J Physiol Pharmacol 2021; 72(5).
2. Durkina AV, Bernikova OG, Mikhaleva NJ, Paderin NM, Sedova KA, Gonotkov MA, Kuzmin VS, Azarov JE. Melatonin pretreatment does not modify extrasystolic burden in the rat ischemia-reperfusion model. J Physiol Pharmacol. 2021; 72(1).
3. Bernikova OG, Vaykshnorayte MA, Ovechkin AO, Sedova KA, Kharin SN, Azarov JE. Preventive Administration of Melatonin Attenuates Electrophysiological Consequences of Myocardial Ischemia. Bull Exp Biol Med. 2020; 169(3):328-331.
4. Bernikova OG, Sedova KA, Durkina AV, Azarov JE. Managing of ventricular reperfusion tachyarrhythmias - focus on a perfused myocardium. J Physiol Pharmacol. 2019; 70(5).

4. Database for the search of the predictors of ventricular tachyarrhythmias in the clinically relevant experimental models of myocardial infarction

The project is devoted to the development of a tool for automatic analysis and storage of intramyocardial electrophysiological data and standard 12-lead ECG signals obtained in the experiments in a porcine myocardial infarction model. The analysis includes processing of the electrical signals and identification of referent points in ECGs and unipolar intramyocardial electrograms in normal and abnormal conditions. The ultimate aim of the project is to create a database of electrophysiological and supplementary physiological data to be used for the search of the predictors of fatal ventricular tachyarrhythmias arising during the episode of acute myocardial ischemia.

Publications:

1. Sbrollini A, Sedova K, Van Dam P, Kautzner J, Morettini M, Burattini L. Point2ecg: An interactive software application for the identification of electrocardiographic fiducial points. In Proceedings of the International Union for Physical and Engineering Sciences in Medicine, Singapore, 12–17 June 2022.

Department of Health Care Disciplines and Population Protection

Sportovců 2311, Kladno, 272 01

Subject of interest

The use of various forms of energy has a long tradition in the treatment of the musculoskeletal system. Sources for physical therapy are natural (solar radiation, radioactive radiation, climatic influences) and artificial (electrical, electromagnetic (non-ionizing and ionizing), acoustic, thermal and mechanical). Human power can also work as a source of energy.

The goal of physical therapy is to induce a physiological response of the organism to the stimulus that affects it, with the aim of activating the organism's own functions and thus achieving a harmonious state. In order to achieve this, it is up to the physician or physiotherapist to select the appropriate energy source for the indication, with the appropriate intensity, length of treatment, and interval between treatments. Robotic rehabilitation is analogous to this principle, with the advantage that its effect does not fluctuate even with prolonged application, the number of repetitions is unlimited and it allows the setting of precise application parameters.

However, there are still many contradictions in the appropriate parameters for each type of treatment, including the use of robots, with recommended methodologies being incomplete or unverified in many places and not always scientifically justified. Dynamically developing technologies offering devices with an wider range of parameters, improving robotic rehabilitation and, in the context of global trends, increasing interest in telerehabilitation are playing an essential role. New possibilities for the use of computer technology are emerging. Technological advancement of medical devices allows to optimize medical and rehabilitation care and to shorten the recovery of patients with various diagnoses. Advanced robotic systems are a great asset for rehabilitation, not only because of the high intensity and homogeneity of the exercises, but also because of the lack of human resources in the health care system. For patient safety and achieving the highest possible treatment efficiency, it is necessary to optimize the recommended treatment procedures using these systems. According to the principles of evidence-based medicine, these procedures must be validated by clinical evaluation. Among other things, we cooperate with the manufacturers of these medical devices within their development, testing and improvement.

What we deal with

• Determining appropriate indications, methodology and treatment schemes for the use of individual robotic systems in clinical practice.
• Development and therapeutic possibilities of new forms of physical therapy.
• The application of robotic procedures in paediatrics with a focus on the rehabilitation of congenital defects.
• The possibilities of phototherapy in musculoskeletal disorders.
• Interaction of ionizing and non-ionizing radiation.
• The development and use of new mechanical aids to increase mobility of the musculoskeletal system.
• Evaluating the impact of the application of innovative assistive technologies in complex rehabilitation care.
• The use of virtual reality in rehabilitation for both diagnostic and therapeutic purposes.
• Objectification and optimization of therapeutic procedures in spa and balneology.

Who we are and with whom we cooperate

A group of academic staff and PhD students from four departments of the Faculty of Biomedical Engineering, academic staff from the Institute for Nanomaterials, Advanced Technologies and Innovations of the Technical University of Liberec, researchers from the Institute of Physiology of the Academy of Sciences, doctors and physiotherapists from clinical departments and technicians from development centres that closely cooperate with the Faculty. These include in particular the Rehabilitation Institute in Kladruby, the THERAP TILIA Clinic, the Rehabilitation of MUDr. Nedělka, Motol University Hospital, the Central Military Hospital - Military University Hospital Prague, the Bulovka University Hospital, the Military Rehabilitation Institute Slapy, the Rehabilitation Hospital Beroun, spa hotels and resorts of the Royal Spa Group, several outpatient workplaces of the EUC Group and others.

Team members

as. Mgr. Martin Brach, odb. as. MUDr. Jan Bříza, CSc., MBA, odb. as. Ing. Yulia Čuprová, Ph.D., odb. as. Ing. Ondřej Gajdoš, Ph.D., odb. as. Mgr. Dita Hamouzová, Ph.D., PhDr. Kristýna Hoidekrová, Ph.D., doc. Ing. Patrik Kutílek, MSc., odb. as. Mgr. Martina Lopotová, Ph.D., Cert. MDT, Ph.D., prof. MUDr. Leoš Navrátil, CSc., MBA, dr.h.c., odb. as. MUDr. Tomáš Nedělka, Ph.D., as. MUDr. Jiří Nedělka,  doc. PhDr. Ing. Jaroslav Průcha, CSc., Ph.D. et. Ph.D., as. Ing. Mgr. Aleš Příhoda, prof. Ing. Aleš Richter, CSc., odb. as. MUDr. Michal Říha, Ph.D., MBA, as. Mgr. Milada Luisa Šedivcová, odb. as. Mgr. Maja Špiritović, Ph.D., as. Ing. Vojtěch Špet.

Research projects

• prof. MUDr. Leoš Navrátil, CSc., MBA, dr.h.c. et al., Ovlivnění příznaků degenerativních onemocnění pohybového aparátu vysokoindukční magnetickou stimulací. AZV, NV16-28784A, 2016 – 2020
• Ing. Mgr. Příhoda Aleš et al., Klinické hodnocení přínosů asistivní robotické rehabilitace při poruchách chůze, SGS20/148/OHK4/2T/17, 2020–2021
• Mgr. Dita Hamouzová Dita et al., Realizace rehabilitační péče v domácím prostředí pod vedením fyzioterapeuta, SGS20/089/OHK4/1T/17, 2020
• Mgr. Dita Hamouzová Dita et al., Zpracování filmů pro odborné vedení klienta při rehabilitační péči prováděné v domácím prostředí. SGS17/205/OHK4/3T/17, 2017 – 2019
• prof. MUDr. Leoš Navrátil, CSc., MBA, dr.h.c. et al., Dlouhověkost bez léků: Popularizace a propagace novinek ve výzkumu nefarmakologických možností ovlivnění zdravotního stavu, spolupříjemce Institut klinické a experimentální medicíny. MŠMT ČR, CZ.1.07/2.3.00/35.0039, 2012 – 2014
• prof. MUDr. Leoš Navrátil, CSc., MBA, dr.h.c. Laboratoř pro rozvoj bakalářského studijního programu Fyzioterapeut (Fyzioterapie pro imobilní nemocné), OPVVV. 2017–2022
• Ing. Mgr. Aleš Příhoda et. al., Centrum lázeňského výzkumu, 2024-2027, OP Spravedlivá transformace – CZ.10.01.01/00/22_001/0000261
• Ing. Vojtěch Špet, Asistivní technologie pro fyzioterapeuty; OPTAK – CZ.01.01.01/01/22_002/0001035, 2024-2025
• prof. MUDr. Ivan Dylevský, DrSc., et. al., Variabilita komplexu struktur kyčelního kloubu v procesu vývoje – SGS21/141/OHK4/2T/17, 2021-2022
• Ing. Mgr. Aleš Příhoda, et. al., Vývoj doporučených léčebných postupů roboticky asistované rehabilitace a telerehabilitace, SGS22/206/OHK4/3T/17; 2022-2024
• doc. PhDr. Ing. Jaroslav Průcha, CSc., Ph.D. et. Ph.D., et.al. Robot pro reedukaci bipedální lokomoce, TA ČR; TH03010299
• doc. Ing. Patrik Kutílek, MSc., Smart Mobility pro děti s postižením – terapie, životní styl a volný čas,  TA ČR; FW04020071
• Ing. Mgr. Aleš Příhoda, et. al., Inovace léčebných metod v lázeňství a balneologii, SGS25/111/OHK4/2T/17; 2025-2026
• Ing. Vojtěch Špet, et. al., Imerzivní virtuální realita pro diagnostiku, monitoring a terapii pacientů léčebně rehabilitační péče, SGS24/155/OHK4/3T/17, 2024-2026

Who are we?

Our team focuses on medical devices in intensive care medicine. We strive to optimize the use of diagnostic and therapeutic equipment in the care of acute patients. We design research studies in intensive care units (ICUs), collect and analyze clinical data and signals. We contribute to the development of new techniques, components, and procedures, and we engage in modeling and simulations for specific patient groups.

A significant part of our activities is dedicated to neonatology. We focus on oxygen dosing in premature newborns, analyze data from specialized medical devices such as regional tissue oxygenation and transcutaneous blood gas measurement, and simulate specific lung diseases.

Our team is also capable of operating a full-body patient simulator and conducting complex patient simulations within a simulated intensive care environment.

Team members:

Ing. Jakub Ráfl, Ph.D. (team leader), doc. Ing. Petr Kudrna, Ph.D., doc. Ing. Martin Rožánek, Ph.D., Ing. Veronika Ráfl Huttová, Ph.D., Ing. Leoš Tejkl, Ph.D., Thomas E Bachman, MSc., Ing. Vít Hlaváč, MUDr. Jana Dorňáková, further PhD, postgraduate and undergraduate students of CTU FBMI.

Our current projects

• PRICO target range
• Neonatal Oxymeter Bias
• Computer model of premature newborns oxygenation
• Models of lung diseases in premature newborns
• System for rapid oxygen delivery to patients
• Heated humidifier for premature newborns

Collaboration

• Neonatal department with intensive care unit, Perinatology Center of the Gynecology and Obstetrics Clinic, Second Faculty of Medicine, Charles University and Motol University Hospital, Prague
• Neonatology Department with ICU/NICU, Department of Gynecology, Obstetrics and Neonatology, General University Hospital and First Faculty of Medicine, Charles University, Prague

Selected Publications

1. Jonáš K, Lamberská T, Nguyen TA, Kudrna P, Plavka R. High‐flow nasal cannula for stabilisation of very premature infants: A prospective observational study. Acta Paediatrica. 2025;114(5):986-91. doi:10.1111/apa.17519.
2. Nguyen TA, Matoušek J, Kuběna A, Resl K, Kudrna P, Dunajová K, Plavka R. Ventilator variables predicting extubation readiness in extremely premature infants with prolonged mechanical ventilation: A retrospective observational study. Pediatric Pulmonology. 2024;59(12):3585-92. doi:10.1002/ppul.27265.
3. Kudrna P. SpO 2 Histograms-Valuable Tools for Assessing Oxygen Saturation Stability of Newborns. In 2024 E-Health and Bioengineering Conference (EHB) 2024 Nov 14 (pp. 1-4). IEEE. doi: 10.1109/EHB64556.2024.10805674.
4. Janota J, Dornakova J, Karadyova V, Brabec R, Rafl-Huttova V, Bachman T, Rozanek M, Rafl J. Evaluation of the impact of oximeter averaging times on automated FiO2 control in routine NICU care: a randomized cross-over study. Frontiers in Pediatrics. 2023;11:1240363. doi: 10.3389/fped.2023.1240363.
5. Tejkl L, Kudrna P, Rafl J, Bachman TE. Reducing the time delay of oxygen transport to the neonate on continuous positive airway pressure support: A bench study. Frontiers in Pediatrics. 2023;11:1141432. doi: 10.3389/fped.2023.1141432.
6. Rafl-Huttova V, Rafl J, Möller K, Bachman TE, Kudrna P, Rozanek M. Statistical Description of SaO2–SpO2 Relationship for Model of Oxygenation in Premature Infants. Electronics. 2022;11(9):1314. doi:10.3390/electronics11091314.
7. Tejkl L, Kudrna P, Rafl J. Hemoglobin ratio as a forgotten parameter for automatic feedback control systems in preterm neonates: Summary of current knowledge. Current Directions in Biomedical Engineering. 2022;8(2):493-496). doi:10.1515/cdbme-2022-1126.
8. Bibbo D, Kijonka J, Kudrna P, Penhaker M, Vavra P, Zonca P. Design and development of a novel invasive blood pressure simulator for patient’s monitor testing. Sensors. 2020;20(1):259. doi:10.3390/s20010259.
9. Bibbo D, Klinkovsky T, Penhaker M, Kudrna P, Peter L, Augustynek M, Kašík V, Kubicek J, Selamat A, Cerny M, Bielcik D. A new approach for testing fetal heart rate monitors. Sensors. 2020;20(15):4139. doi:10.3390/s20154139.
10. Bachman TE, Rafl J. Response by an automated inspired oxygen control system to hypoxemic episodes: Assessment of damping. Clinician and Technology. 2018;48(2):41-5.

In  Nanosensors for Biomedicine grup we focus on the application nanomaterials and methods for development of new methods in omicroscopy. The lab is equipped with a state-of-the-art time-resolved confocal microscope for analyzing the optical properties of nanoparticles and molecules, as well as an atomic force microscope used for studying nanoparticles and cells, along with other specialized instruments. 

Current research topics include:

• Laser-Induced Fluorescence in Ophthalmic Materials
• Biosensor applications based on nanostructured diamond layers
• Algorithmic sorting of signals from nerve cells

Research Topics

Laser-Induced Fluorescence in Ophthalmic Materials

We focuse on studying laser-induced fluorescence in intraocular lenses (IOL). The results may contribute to improved modification of the optical properties of polymeric IOL materials directly inside the patient's eye.

We characterizes fluorophores generated during interactions between femtosecond lasers and polymeric materials (e.g., hydrogels and acrylates). The goal is to understand how these fluorophores affect the optical and structural properties of the materials and how these changes could be used to enhance IOL functionality. Femtosecond-laser tuning of implanted lenses could provide safe, in-situ optical correction without further surgery. Our findings can help in developing the way for next-generation IOLs with adjustable optical power, directly addressing a significant clinical need.

Plasmon-Enhanced Superresolution Microscopy and Bioimaging

We are working on a novel approach for nanoscale spatial control of light using assemblies of plasmonic nanostructures. This method aims to improve visualization of densely packed biomolecules and their dynamic behavior.

Metallic nanostructures can significantly enhance optical signals due to collective electromagnetic excitations known as plasmons. The interaction between a fluorophore and a plasmonic structure can markedly increase photon emission. Recent advances in superresolution microscopy have uncovered previously inaccessible phenomena related to plasmonic enhancement. Our research focuses on controlling these shifts by tuning the enhancement mechanisms, the fluorophore–plasmon separation, and their dynamic interactions.

Biosensor Applications Based on Nanostructured Diamond Layers

The lab develops systems using microelectrode arrays (MEA) with nanodiamond surfaces to monitor the electrical and neurochemical activity of nerve cells. Most existing systems are limited to monitoring only neuro-electrical activity, without tracking neurochemical signals like neurotransmitters or metabolites. Our research focuses on developing MEAs based on nanostructured boron-doped diamond (BDD), a material that is mechanically stable, biocompatible, and supports low-noise recording. We also work on advanced signal processing techniques.

Group members

• Václav Petrák, Ph.D. 
• Assoc. Prof. . Vladimíra Petráková, Ph.D.
• Khalilullah Umar, MSc (Ph.D. Candidate)
• Ing. Adéla Mádlová (Ph.D. Candidat)

Laboratory Equipment

MICROTIME 200 Microscope

• Time-resolved confocal fluorescence microscope
• Capable of detecting signals from individual molecules
• Supports Time-Correlated Single Photon Counting (TCSPC) for studying dynamic processes using FCS and FLIM methods

NANOWizard Sense Atomic Force Microscope

• Integrated with a confocal fluorescence microscope for simultaneous monitoring of fluorescence and surface morphology
• Bio-sample chamber enables live cell culture measurements

Andor iXon 888 Ultra

• Ultra-fast Electron Multiplying CCD (EMCCD) camera
• Enables ultra-sensitive detection across a wide field of view at 26 fps

Microelectrode Array Measurement System

• Multichannel Systems MEA1060 for recording from neurons and other electrically active tissues using 60 independent electrodes

Other Equipment

• Inverted microscope
• Nano-positioning stage
• FRITSCH P-23 mini-mill
• GEM 532nm laser
• Elmasonic-S 15/H ultrasonic cleaner

Selected Publications

1. Hektrdla et. al (2025) Optimized molecule detection in localization microscopy with selected false positive probability, Nature Communications, 16(601)
2. Klempíř et al. (2020). Application of Spike Sorting Algorithm to Neuronal Signals Originated from Boron Doped Diamond Micro-Electrode Arrays. Physiological Research, 69(3), 529–536.
3. Krůšek et al. (2019). Molecular Functionalization of Planar Nanocrystalline and Porous Nanostructured Diamond.... physica status solidi (b), 256(3), 1800424.
4. Jakubcová et al. (2018). Neuron Adhesion on Diamond: Competition between Polymer Treatment and Surface Morphology. Advanced Engineering Materials, 20(9), 1800182.
5. Lukowski et al. (2018). Fluorescent Nanodiamonds as Vehicles for Non-Coding Regulatory RNA Delivery. Journal of Biomedical Nanotechnology, 14(5), 946–958.
6. Havlík, Petráková (2018). Rapid Isotropic Irradiation of Nanoparticles by In Situ Generated Ions. Nature Communications, 9(1), 1–10.

Collaborations

The lab collaborates with top institutions in the Czech Republic and abroad:

Czech Republic: 

• Institute of Physics (CAS), 
• Institute of Organic Chemistry and Biochemistry (CAS),
• J. Heyrovský Institute of Physical Chemistry (CAS), 
• Institute of Microbiology (CAS), Faculty of Science, Charles University

International

• University of Rochester (USA)
• Hasselt University (Belgium), 
• Freie Universität Berlin (Germany)

Research Projects

Current projects

• INTER-EXCELLENCE II (2024-2027): Fluorescence of Polymers Treated by a Femtosecond Laser
• SGS (2025-2026): Structutal Analysis of Laser-Modified Hydrogels
• SGS (2024-2025): High resolution near-infrared imaging with DNA-Paint

Previous projects

• GAČR (2017–2019) Diamond based microelectrode arrays for dual mode neural recording 
• SGS (2023-2024): Evaluation of psychoactive substances using neuronal networks